Tsina I W, Matin S B
J Pharm Sci. 1981 Aug;70(8):858-60. doi: 10.1002/jps.2600700805.
A specific and sensitive high-performance liquid chromatographic method for the analysis of oxfendazole in cow milk is described. Oxfendazole was extracted from milk using a mixture of acetone and chloroform under alkaline conditions. The solvents were evaporated, and the oily residue was purified by hexane-acetonitrile partition and acid-base extraction. The residue obtained after cleanup was redissolved in methanol for chromatographic analysis. Chromatography was performed on a reversed-phase column with acetonitrile-water as the mobile phase. As low as 0.005 microgram of oxfendazole/g can be measured by this method using 50 g of milk. The method was applied to measure oxfendazole in the milk of a cow given an oral 5-mg/kg dose.
描述了一种用于分析牛奶中奥芬达唑的特异性和灵敏的高效液相色谱法。在碱性条件下，使用丙酮和氯仿的混合物从牛奶中提取奥芬达唑。将溶剂蒸发，油性残留物通过己烷 - 乙腈分配和酸碱萃取进行纯化。净化后得到的残留物重新溶解在甲醇中进行色谱分析。在反相柱上进行色谱分析，以乙腈 - 水作为流动相。使用50克牛奶，该方法可测定低至0.005微克/克的奥芬达唑。该方法用于测定口服5毫克/千克剂量的奶牛的牛奶中的奥芬达唑。
