无色杆菌生产 l-色氨酸。
l-Tryptophan Production by Achromobacter liquidum.
机构信息
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka 532, Japan.
出版信息
Appl Environ Microbiol. 1983 Jul;46(1):1-5. doi: 10.1128/aem.46.1.1-5.1983.
Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produced most efficiently by shaking the cells at 37 degrees C in a reaction mixture containing 60 mg of l-serine per ml, 60 mg of indole per ml, and 0.5 mM pyridoxal phosphate. After 3 days, 96 mg of l-tryptophan per ml was formed, and l-tryptophan was easily isolated to 85.4% yield by concentration of the reaction mixture.
研究了从无色杆菌(Achromobacter liquidum)生产色氨酸酶以及将 l-丝氨酸和吲哚转化为 l-色氨酸的条件。在 30°C 下，通过在含有糊精、酵母提取物、l-色氨酸和 l-谷氨酸的培养基中振荡培养，可以产生高达 0.750 U/ml（降解）和 0.294 U/ml（合成）的酶。通过在含有 60mg/ml l-丝氨酸、60mg/ml 吲哚和 0.5mM 吡哆醛磷酸的反应混合物中在 37°C 下振荡细胞，最有效地生产 l-色氨酸。3 天后，每毫升形成 96mg 的 l-色氨酸，通过浓缩反应混合物，l-色氨酸很容易以 85.4%的产率分离得到。
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