Gruss A, Novick R
J Bacteriol. 1986 Mar;165(3):878-83. doi: 10.1128/jb.165.3.878-883.1986.
Elimination of plasmids from regenerating S. aureus protoplasts occurred when the regeneration medium contained sucrose but not when it contained sodium succinate. This difference was caused by the occurrence of cell division prior to regeneration of the cell wall on sucrose but not on succinate. Coexisting compatible plasmids were cured independently; coexisting incompatible plasmids were cured jointly. These results support the hypothesis that plasmid pools exist as physically sequestered units in protoplasts and that curing is a consequence of the segregation of such units during abnormal division of wall-less organisms.
当再生培养基含有蔗糖时,金黄色葡萄球菌原生质体再生过程中质粒会消除,而当含有琥珀酸钠时则不会。这种差异是由于在蔗糖上细胞壁再生之前发生了细胞分裂,而在琥珀酸钠上则没有。共存的相容性质粒会独立消除;共存的不相容性质粒会共同消除。这些结果支持了以下假设:质粒库在原生质体中以物理隔离的单位存在,并且消除是无壁生物体异常分裂过程中这些单位分离的结果。