Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Clin Exp Dent Res. 2024 Dec;10(6):e70020. doi: 10.1002/cre2.70020.
Gingivitis is the initial stage of periodontitis, one of the most common oral diseases and the primary cause of tooth loss. This study aims to evaluate the effect of toothpaste containing 2% zinc citrate on gingival health and the abundance of three bacteria related to gingivitis and periodontitis.
Eleven volunteers with the same oral health status were randomly assigned to the treatment (n = 5) and control (n = 6) groups. The control group used fluoride toothpaste, while the treatment group used fluoride toothpaste supplemented with 2% zinc citrate for 3 months. The plaque index, gingival index, and bleeding index were measured at baseline (0 day), 3 weeks, and 3 months. Dental plaque from four areas of the mouth (FDI criteria) was collected at the same timepoints. A total of 132 dental plaque samples were analyzed using quantitative PCR (qPCR) to monitor the abundance of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia.
Toothpaste containing 2% zinc citrate significantly lowered the gingival index and reduced gum bleeding but did not affect the plaque index. It also reduced the total abundance of the three bacteria related to gingivitis and periodontitis in dental plaque over a long-term period.
Toothpaste with 2% zinc citrate persistently improves gingival health and reduces the presence of gingivitis-associated bacteria in dental plaque.
Chinese Clinical Trial Registry (Clinical trial registration no.: ChiCTR1900020592) (09/01/2019).
牙龈炎是牙周炎的初始阶段，是最常见的口腔疾病之一，也是牙齿脱落的主要原因。本研究旨在评估含有 2%柠檬酸锌的牙膏对牙龈健康以及与牙龈炎和牙周炎相关的三种细菌丰度的影响。
11 名口腔健康状况相同的志愿者被随机分配到治疗组（n=5）和对照组（n=6）。对照组使用含氟牙膏，治疗组使用含氟牙膏加 2%柠檬酸锌补充剂，持续 3 个月。在基线（0 天）、3 周和 3 个月时测量菌斑指数、牙龈指数和出血指数。同时在口腔四个区域（FDI 标准）采集牙菌斑。使用定量 PCR（qPCR）共分析了 132 个牙菌斑样本，以监测伴放线放线杆菌、牙龈卟啉单胞菌和福赛坦纳菌的丰度。
含有 2%柠檬酸锌的牙膏显著降低了牙龈指数和减少了牙龈出血，但对菌斑指数没有影响。它还在长期内降低了牙菌斑中与牙龈炎和牙周炎相关的三种细菌的总丰度。
含有 2%柠檬酸锌的牙膏可持续改善牙龈健康并减少牙菌斑中与牙龈炎相关的细菌。
中国临床试验注册中心（临床试验注册号：ChiCTR1900020592）（2019 年 9 月 1 日）。
