Sino-Danish Centre for Education and Research, University of Chinese Academy of Sciences, Beijing 100049, PR China.
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, PR China.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5217-5225. doi: 10.1099/ijsem.0.004395. Epub 2020 Aug 20.
Two novel Gram-stain-negative, aerobic and non-motile rods bacteria, designated TQ8S and ZH2S, were isolated from salt marsh sediment collected from the Tibetan Plateau. Strain TQ8S was found to grow at 10-40 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 8.0-9.0) and in the presence of 2-12 % (w/v) NaCl (optimum, 6-8 %). Strain ZH2S was found to grow at 15-40 °C (optimum, 30 °C), pH 7.0-10.0 (optimum pH 9.0) and in the presence of 2-10 % (w/v) NaCl (optimum, 4-6 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains TQ8S and ZH2S shared 99.07 % sequence similarity between each other and were affiliated with the genus , sharing 97.48 % and 97.41 % of sequence similarity to their closest neighbour Esulfide1, respectively. DNA-DNA hybridization analyses showed 61.0 % relatedness between strains TQ8S and ZH2S. The average nucleotide identity and the average amino acid identity values between the two genomes were 92.33 and 92.84 %, respectively. The values between the two strains and their close phylogenetic relatives were all below 95 %. The major respiratory quinones of strain TQ8S were Q-9 and Q-8, while that of ZH2S was Q-9. The main fatty acids shared by the two strains were C 6c and/or C 7c, C 6c and/or C 7c, C and C 3-OH. Strain ZH2S can be distinguished from TQ8S by a higher proportion of C cyclo 8. The G+C content of the genomic DNA of strains TQ8S and ZH2S were 57.20 and 57.14 mol%, respectively. On the basis of phenotypic distinctiveness and phylogenetic divergence, the two isolates are considered to represent two novel species of the genus , for which the names sp. nov (type strain TQ8S=KCTC 62530=CICC 24572) and sp. nov (type strain ZH2S=KCTC 62531=CICC 24505) are proposed.
从青藏高原盐沼沉积物中分离到两株革兰氏阴性、需氧、非运动的棒状细菌，分别命名为 TQ8S 和 ZH2S。菌株 TQ8S 生长的温度范围为 10-40°C（最适温度为 30°C），pH 值范围为 6.0-11.0（最适 pH 值为 8.0-9.0），能在 2-12%（w/v）NaCl（最适浓度为 6-8%）的环境中生长。菌株 ZH2S 生长的温度范围为 15-40°C（最适温度为 30°C），pH 值范围为 7.0-10.0（最适 pH 值为 9.0），能在 2-10%（w/v）NaCl（最适浓度为 4-6%）的环境中生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 TQ8S 和 ZH2S 彼此之间的序列相似度为 99.07%，属于 属，与最接近的近缘种 Esulfide1 的序列相似度分别为 97.48%和 97.41%。DNA-DNA 杂交分析表明，菌株 TQ8S 和 ZH2S 之间的相关性为 61.0%。两个基因组之间的平均核苷酸同一性和平均氨基酸同一性值分别为 92.33%和 92.84%。这两个菌株与它们的近亲的数值均低于 95%。菌株 TQ8S 的主要呼吸醌为 Q-9 和 Q-8，而 ZH2S 的主要呼吸醌为 Q-9。两个菌株共有的主要脂肪酸为 C 6c 和/或 C 7c、C 6c 和/或 C 7c、C 和 C 3-OH。菌株 ZH2S 可通过较高比例的 C 环 8 与 TQ8S 区分开来。菌株 TQ8S 和 ZH2S 的基因组 DNA 的 G+C 含量分别为 57.20%和 57.14%。基于表型特征和系统发育差异，这两个分离株被认为代表了 属的两个新种，分别命名为 sp. nov（TQ8S 株=KCTC 62530=CICC 24572）和 sp. nov（ZH2S 株=KCTC 62531=CICC 24505）。
