sp. nov. and sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau.

Two novel Gram-stain-negative, aerobic and non-motile rods bacteria, designated TQ8S and ZH2S, were isolated from salt marsh sediment collected from the Tibetan Plateau. Strain TQ8S was found to grow at 10-40 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 8.0-9.0) and in the presence of 2-12 % (w/v) NaCl (optimum, 6-8 %). Strain ZH2S was found to grow at 15-40 °C (optimum, 30 °C), pH 7.0-10.0 (optimum pH 9.0) and in the presence of 2-10 % (w/v) NaCl (optimum, 4-6 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains TQ8S and ZH2S shared 99.07 % sequence similarity between each other and were affiliated with the genus , sharing 97.48 % and 97.41 % of sequence similarity to their closest neighbour Esulfide1, respectively. DNA-DNA hybridization analyses showed 61.0 % relatedness between strains TQ8S and ZH2S. The average nucleotide identity and the average amino acid identity values between the two genomes were 92.33 and 92.84 %, respectively. The values between the two strains and their close phylogenetic relatives were all below 95 %. The major respiratory quinones of strain TQ8S were Q-9 and Q-8, while that of ZH2S was Q-9. The main fatty acids shared by the two strains were C 6c and/or C 7c, C 6c and/or C 7c, C and C 3-OH. Strain ZH2S can be distinguished from TQ8S by a higher proportion of C cyclo 8. The G+C content of the genomic DNA of strains TQ8S and ZH2S were 57.20 and 57.14 mol%, respectively. On the basis of phenotypic distinctiveness and phylogenetic divergence, the two isolates are considered to represent two novel species of the genus , for which the names sp. nov (type strain TQ8S=KCTC 62530=CICC 24572) and sp. nov (type strain ZH2S=KCTC 62531=CICC 24505) are proposed.