Evaluation of a strain from plants as a novel promising probiotic in dental caries management.

BACKGROUND

Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified AFS098024 as a probiotic candidate isolated from plants.

METHODS

The safety of was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. biofilm model derived from the saliva of caries-free and caries-active donors and rat caries model were used to assess the efficacy of in caries prevention and treatment.

RESULTS

was safe as a probiotic candidate and it could integrate with biofilm model. It significantly reduced the biomass and lactate production of biofilms from caries-active donors and disrupted biofilm structures. effectively reduced the severity of carious lesions in rat molars, regardless of the inoculation sequence. Molars pretreated or treated with demonstrated notably higher enamel volumes. Additionally, colonization of rat molars by persisted for 6 weeks.

CONCLUSION

The strain used in this study holds promise as a probiotic for inhibiting dental caries, both and .