Jiang Qipeng, Wang Yong, Yu Jiamin, Wang Jinfeng, Guo Shiping, Liu Dongyang, Yu Xiangwen, Jiang Lianqiang, Long Gang, Xi Daojiang, Chen Shuhong, Wang Yue, Ding Wei
College of Plant Protection, Southwest University, Chongqing, China.
Liangshan Branch of Sichuan Tobacco Company, Xichang, China.
Front Microbiol. 2024 Oct 22;15:1415700. doi: 10.3389/fmicb.2024.1415700. eCollection 2024.
Rhizosphere microbial communities strongly affect outbreaks of root-knot nematode (RKN) disease. However, little is known about the interactions among fungi, bacteria and RKN. The bacterial and fungal community compositions in the rhizospheres of four representative tobacco varieties, both resistant and susceptible to RKN, were characterized using 16S rRNA gene sequencing for bacteria and internal transcribed spacer gene sequencing for fungi. Our findings revealed that the fungi played crucial roles in facilitating the cross-kingdom and symbiotic fungal-bacterial interactions to suppress RKN. Moreover, our investigation suggested as a potential microbial antagonist against RKN based on its enhanced presence in RKN-resistant tobacco genotypes, and the relative abundance of was 34.49% greater in the rhizosphere of resistant tobacco than that of susceptible tobacco significantly. Notably, the richness of fungal community enhanced tobacco's microbe-associated resistance to RKN through the positive regulation of the richness and diversity of bacterial community and the relative abundance of . This study underscores the critical role of the fungus-dominated fungal-bacterial community in bolstering tobacco resistance against RKN. The potential antagonistic role of presents promising avenues for innovative RKN management strategies.
根际微生物群落强烈影响根结线虫（RKN）病害的爆发。然而，关于真菌、细菌和根结线虫之间的相互作用却知之甚少。利用细菌的16S rRNA基因测序和真菌的内转录间隔区基因测序，对4个对根结线虫具有抗性和敏感性的代表性烟草品种根际的细菌和真菌群落组成进行了表征。我们的研究结果表明，真菌在促进跨界和共生真菌 - 细菌相互作用以抑制根结线虫方面发挥了关键作用。此外，我们的调查表明，基于其在抗根结线虫烟草基因型中的存在增加， 作为一种潜在的针对根结线虫的微生物拮抗剂，并且在抗性烟草根际中的相对丰度比易感烟草显著高34.49%。值得注意的是，真菌群落的丰富度通过对细菌群落的丰富度、多样性以及 的相对丰度的正向调节，增强了烟草与微生物相关的对根结线虫的抗性。本研究强调了以真菌为主导的真菌 - 细菌群落对增强烟草对根结线虫抗性的关键作用。 的潜在拮抗作用为创新的根结线虫管理策略提供了有前景的途径。
