Goldy Jordan N, Youker Robert T
Department of Biology, Emory University, Atlanta, Georgia, United States.
Department of Biology , Western Carolina University, Cullowhee, North Carolina, United States.
MicroPubl Biol. 2024 Oct 21;2024. doi: 10.17912/micropub.biology.001312. eCollection 2024.
Cells have evolved an elaborate network of folding and degradation pathways to maintain the native state of proteins. If these pathways are disrupted (e.g., mutation) or their capacity is exceeded then protein aggregates form in cells. Cells sequester these aggregated proteins into aggresome or aggresome-like bodies as a protective mechanism. In this study, we co-expressed two model misfolded proteins in HEK293 cells and measured aggresome formation upon proteasomal inhibition. We observed spatial differences in early time points of aggresome formation upon co-expression of the misfolded proteins compared to individual expression in cells.
细胞已经进化出一套复杂的折叠和降解途径网络，以维持蛋白质的天然状态。如果这些途径被破坏（例如，发生突变）或超出其能力范围，那么细胞内就会形成蛋白质聚集体。细胞将这些聚集的蛋白质隔离到聚集体或类聚集体中，作为一种保护机制。在本研究中，我们在人胚肾293细胞中共表达了两种模型错误折叠蛋白，并在蛋白酶体抑制后测量聚集体的形成。我们观察到，与细胞中单独表达相比，共表达错误折叠蛋白时，聚集体形成的早期时间点存在空间差异。
