Bueno Juan M, Martínez-Ojeda Rosa M, Fernández Enrique J, Feldkaemper Marita
Laboratorio de Óptica, Instituto Universitario de Investigación en Óptica y Nanofísica, Universidad de Murcia, Murcia, Spain.
Section of Neurobiology of the Eye, Institute for Ophthalmic Research, Tuebingen, Germany.
Front Med (Lausanne). 2024 Oct 22;11:1462024. doi: 10.3389/fmed.2024.1462024. eCollection 2024.
Visual deprivation causes enhanced eye growth and the development of myopia, which is associated with a change in the arrangement of collagen fibers within the sclera. A second harmonic generation (SHG) microscope has been used to image the collagen fibers of unstained scleral punches from the posterior part of chicken eyes. We aimed to analyze the fibrous scleral tissue and quantify the changes in collagen organization in relation to the extent of induced deprivation myopia. The scleral architecture was assessed with the Radon transform (RT) through the parameter called structural dispersion (SD) that provides an objective tool to quantify the level of organization of the collagen network. We found that final refraction and axial length changes were linearly correlated. However, no significant differences in scleral thickness were found for different amounts of induced myopia. In contrast, a significant correlation between SD and refraction was demonstrated, ranging from a non-organized (in the control sclerae) to a quasi-aligned distribution (with a dominant direction of the fibers, in the sclera of myopic chicks). These findings demonstrate a remodeling process of the scleral collagen associated with myopia progression that can be measured accurately combining SHG imaging microscopy and RT algorithms.
视觉剥夺会导致眼球过度生长并引发近视，这与巩膜内胶原纤维排列的改变有关。二次谐波产生（SHG）显微镜已被用于对鸡眼后部未染色巩膜组织块中的胶原纤维进行成像。我们旨在分析纤维状巩膜组织，并量化与诱导性剥夺性近视程度相关的胶原组织变化。通过称为结构离散度（SD）的参数，利用拉东变换（RT）评估巩膜结构，该参数为量化胶原网络的组织水平提供了一种客观工具。我们发现最终屈光度和眼轴长度变化呈线性相关。然而，对于不同程度的诱导性近视，未发现巩膜厚度有显著差异。相反，SD与屈光度之间存在显著相关性，范围从无序状态（对照巩膜）到准排列分布（纤维有主导方向，在近视雏鸡的巩膜中）。这些发现表明，与近视进展相关的巩膜胶原重塑过程可以通过结合SHG成像显微镜和RT算法进行准确测量。
