Yu Junwei, Zhang Yunpeng, Clements Kelsey, Chen Nannan, Griffith Leslie C
Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA.
These authors contributed equally.
Res Sq. 2024 Oct 21:rs.3.rs-5021271. doi: 10.21203/rs.3.rs-5021271/v1.
Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we developed genetically-encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy, to enable single-vesicle resolution with confocal microscopy.
神经元致密核心囊泡（DCV）储存并释放多种神经调质、营养因子和生物胺。对单个DCV的分析在很大程度上仅能通过电子显微镜进行，这使得理解货物分选和DCV异质性变得困难。为了解决这些局限性，我们开发了用于DCV的基因编码标记物，其可与标准免疫组织化学和扩展显微镜结合使用，以实现共聚焦显微镜下的单囊泡分辨率。
