Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui, China.
Institute of Respiratory Diseases, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui, China.
Can Respir J. 2024 Oct 28;2024:4505905. doi: 10.1155/2024/4505905. eCollection 2024.
Vitamin D deficiency elevates the risk of chronic obstructive pulmonary disease (COPD) patients. Iron parameters elevation and glutathione peroxidase 4 (GPX4) reduction are involved in the process of COPD. The goal is to explore the associations of vitamin D with GPX4 and iron parameters in COPD patients through a case-control study. COPD patients and control subjects were enrolled. Serum samples and lung tissues were collected. Serum vitamin D and iron levels and pulmonary ferritin and GPX4 expressions were determined. In addition, human pulmonary epithelial cells (BEAS-2B) were incubated with 1,25(OH)D (100 nM), the active form of vitamin D3. Then, vitamin D receptor (VDR) and nuclear factor (erythroid-derived 2)-like 2 (Nrf-2) signaling were detected. In patients with COPD, serum 25-hydroxyvitamin D (25(OH)D) decreased, and iron and ferritin levels in serum and lung tissues increased. Furthermore, pulmonary expression of GPX4 was reduced. Correlative analyzes indicated that lung function was inversely correlated with iron parameters and positively correlated with GPX4. The results showed that serum 25(OH)D deficiency was associated with an elevation in serum iron parameters and a reduction in pulmonary GPX4. In addition, VDR- and Nrf-2-positive lung nuclei were decreased in COPD patients than in control subjects. In patients with COPD, the results indicated a positive relationship between VDR and Nrf-2. Further analysis revealed that Nrf-2-positive nuclei were negatively correlated with iron parameters. experiments found that 1,25(OH)D treatment activated VDR signaling and elevated the expression of Nrf-2 and GPX4 in BEAS-2B cells. Vitamin D deficiency is positively associated with GPX4 reduction and iron parameters elevation in COPD patients. It is recommended to explore the role of vitamin D supplementation in the progression of COPD.
维生素 D 缺乏会增加慢性阻塞性肺疾病（COPD）患者的风险。铁参数升高和谷胱甘肽过氧化物酶 4（GPX4）减少参与了 COPD 的发生过程。本研究通过病例对照研究旨在探讨 COPD 患者维生素 D 与 GPX4 和铁参数的相关性。纳入 COPD 患者和对照受试者，采集血清样本和肺组织，检测血清维生素 D 和铁水平以及肺组织铁蛋白和 GPX4 表达。此外，用 1,25(OH)D（100nM），维生素 D3 的活性形式，孵育人肺上皮细胞（BEAS-2B），然后检测维生素 D 受体（VDR）和核因子（红系衍生 2）样 2（Nrf-2）信号。在 COPD 患者中，血清 25-羟维生素 D（25(OH)D）降低，血清和肺组织中铁和铁蛋白水平升高，肺组织中 GPX4 的表达降低。相关性分析表明，肺功能与铁参数呈负相关，与 GPX4 呈正相关。结果表明，血清 25(OH)D 缺乏与血清铁参数升高和肺 GPX4 降低有关。此外，与对照组相比，COPD 患者的 VDR 和 Nrf-2 阳性肺核减少。在 COPD 患者中，结果表明 VDR 和 Nrf-2 之间存在正相关。进一步分析显示，Nrf-2 阳性核与铁参数呈负相关。实验发现，1,25(OH)D 处理激活了 VDR 信号通路，增加了 BEAS-2B 细胞中 Nrf-2 和 GPX4 的表达。维生素 D 缺乏与 COPD 患者 GPX4 降低和铁参数升高呈正相关。建议探索维生素 D 补充在 COPD 进展中的作用。
