Emedic Salud, Lima, Peru.
Pediatric Health National Institute, Lima, Peru.
J Immunol Res. 2024 Aug 20;2024:8657483. doi: 10.1155/2024/8657483. eCollection 2024.
The Peruvian Immunoblot panel, together with traditional skin prick tests (SPT), are widely used in vitro allergy tests in Peru. In addition to this, Peruvian allergists are increasingly adopting multiplex tests such as the ALEX-2 (Macro Array Diagnostics). Previous studies have revealed limited agreement between Immunoblot and SPT results. Therefore, our study aimed to evaluate the concordance between these three tests in patients with allergic rhinitis (AR) in a private center in Arequipa, Peru.
We enrolled 35 patients, including children over 3 years and adults, with AR. Clinical and demographic data were collected, and patients underwent allergic sensitization testing using the Immunoblot Peruvian panel (32 allergens), ALEX-2 (295 allergens), and SPT (12 allergens). Concordance was calculated using Cohen's kappa coefficient and analyzed with IBM SPSS V26.
Among the patients, 34.3% exhibited moderate-to-severe persistent AR, and 14.3% had asthma. Additionally, 85.7% reported a family history of AR. Sensitization rates varied notably between the SPT and ALEX-2, particularly for olive pollen (34.3% vs. 17.4%), Blomia tropicalis (11.4% vs. 17.1%), and grasses (11.4% vs. 28.5%). Remarkably, these allergens were not included in the Peruvian Immunoblot panel. Concordance analysis included seven allergens and showed significant concordance between ALEX-2 and SPT for five allergens, between Immunoblot and SPT for two allergens, and between ALEX-2 and Immunoblot for two allergens.
This preliminary study shows us a better concordance between ALEX-2 and SPT rather than between Immunoblot and SPT.
秘鲁免疫印迹面板与传统皮肤点刺试验（SPT）一起，在秘鲁被广泛用于体外过敏试验。除此之外，秘鲁过敏症专家越来越多地采用多重检测，如 ALEX-2（宏阵列诊断）。以前的研究表明免疫印迹与 SPT 结果之间的一致性有限。因此，我们的研究旨在评估在秘鲁阿雷基帕的一个私人中心，这三种检测方法在过敏性鼻炎（AR）患者中的一致性。
我们招募了 35 名患者，包括 3 岁以上的儿童和成人，他们都患有 AR。收集临床和人口统计学数据，并使用秘鲁免疫印迹面板（32 种过敏原）、ALEX-2（295 种过敏原）和 SPT（12 种过敏原）对患者进行过敏敏感测试。使用 Cohen 的 kappa 系数计算一致性，并使用 IBM SPSS V26 进行分析。
在患者中，34.3%为中重度持续性 AR，14.3%患有哮喘。此外，85.7%报告有 AR 家族史。SPT 和 ALEX-2 之间的致敏率差异显著，特别是橄榄花粉（34.3%比 17.4%）、布鲁米亚热带（11.4%比 17.1%）和草类（11.4%比 28.5%）。值得注意的是，这些过敏原并未包含在秘鲁免疫印迹面板中。一致性分析包括 7 种过敏原，结果显示 ALEX-2 和 SPT 之间有 5 种过敏原具有显著一致性，免疫印迹和 SPT 之间有 2 种过敏原具有一致性，ALEX-2 和免疫印迹之间有 2 种过敏原具有一致性。
这项初步研究表明，ALEX-2 和 SPT 之间的一致性要好于免疫印迹和 SPT 之间的一致性。
