Gould E A, Buckley A, Barrett A D, Cammack N
J Gen Virol. 1986 Mar;67 ( Pt 3):591-5. doi: 10.1099/0022-1317-67-3-591.
The capacity of monoclonal antibodies to protect mice passively against yellow fever (YF) virus infection was investigated. Both neutralizing (54K-specific) and non-neutralizing (54K- and 48K-specific) antibodies protected mice against challenge with the RMP substrain of YF virus. Average survival times of mice inoculated intracerebrally with a standard lethal dose of YF virus differed according to the strain used: thus mice inoculated with the most neurovirulent viruses, FNV and Asibi, survived for 6 X 50 and 7 X 65 days respectively, and those with RMP virus survived for 15 X 75 days. The capacity of antibodies to protect mice passively against virus challenge was directly related to virus neurovirulence. Possible mechanisms and the significance of protection by antibodies against non-structural proteins that do not mediate neutralization, are discussed.
研究了单克隆抗体被动保护小鼠免受黄热病（YF）病毒感染的能力。中和性（54K特异性）和非中和性（54K和48K特异性）抗体均能保护小鼠免受YF病毒RMP亚株的攻击。用标准致死剂量的YF病毒脑内接种小鼠后的平均存活时间因所用病毒株而异：因此，接种神经毒性最强的病毒FNV和阿西比的小鼠分别存活了6×50天和7×65天，而接种RMP病毒的小鼠存活了15×75天。抗体被动保护小鼠免受病毒攻击的能力与病毒神经毒性直接相关。文中讨论了抗体针对不介导中和作用的非结构蛋白发挥保护作用的可能机制及意义。
