Buckley A, Gould E A
J Gen Virol. 1985 Dec;66 ( Pt 12):2523-31. doi: 10.1099/0022-1317-66-12-2523.
Monoclonal and polyclonal antibodies with known specificity for either the 54K envelope glycoprotein or the 48K non-structural glycoprotein of yellow fever (YF) virus-infected cells were studied in plaque reduction neutralization tests. Viruses employed in the tests comprised wild-type and vaccine strains of YF and a selection of other flaviviruses. Of 17 monoclonal antibodies examined, six of the 54K-specific antibodies neutralized at least one YF preparation. Both vaccine and wild-type YF viruses varied in their susceptibility to neutralization and there were also differences between individual 17D vaccine strains. The monoclonal antibodies produced a range of titres with the different viruses, the most potent, 864, leaving no non-neutralizable fraction. Addition of anti-globulin, complement or other antibodies did not affect the results. YF-neutralize antibodies which bound to other flaviviruses did not necessarily neutralize them; hence, neutralization could be defined as either homotypic, heterotypic or both homotypic and heterotypic. A polyclonal antiserum and a broadly reacting monoclonal antibody produced almost identical neutralization results in tests with wild-type YF viruses. In contrast, the polyclonal antiserum produced higher titres with vaccine strains of YF. In mouse passive protection experiments on the other hand, the monoclonal antibody did not differentiate between these viruses.
在蚀斑减少中和试验中，研究了对黄热病（YF）病毒感染细胞的54K包膜糖蛋白或48K非结构糖蛋白具有已知特异性的单克隆抗体和多克隆抗体。试验中使用的病毒包括YF的野生型和疫苗株以及一些其他黄病毒。在检测的17种单克隆抗体中，6种针对54K的特异性抗体至少中和了一种YF制剂。疫苗株和野生型YF病毒对中和的敏感性各不相同，而且各17D疫苗株之间也存在差异。单克隆抗体对不同病毒产生了一系列效价，其中最有效的效价为864，没有留下不可中和的部分。添加抗球蛋白、补体或其他抗体不影响结果。与其他黄病毒结合的YF中和抗体不一定能中和这些病毒；因此，中和可定义为同型、异型或同型和异型兼具。在对野生型YF病毒的试验中，一种多克隆抗血清和一种广泛反应的单克隆抗体产生了几乎相同的中和结果。相比之下，多克隆抗血清对YF疫苗株产生的效价更高。另一方面，在小鼠被动保护实验中，单克隆抗体无法区分这些病毒。
