Tuleubayev Berik, Kamyshanskiy Yevgeniy, Saginova Dina, Akhmetova Saule, Vinokurov Vladimir, Koshanova Amina, Kossilova Yekaterina
Department of Surgical Diseases Karaganda Medical University Karaganda Kazakhstan.
Pathology Unit of the University Clinic Karaganda Medical University Karaganda Kazakhstan.
J Exp Orthop. 2024 Nov 5;11(4):e70067. doi: 10.1002/jeo2.70067. eCollection 2024 Oct.
The present study aimed to evaluate and compare the effect of nanofibrillated cellulose (NFC)-based composite with dicalcium phosphate dihydrate and an autologous blood clot (ABC) on the formation of new bone tissue in vivo by histological and histomorphometric assessment.
A total of 72 rats with created femoral defects (2 mm) were used. The rats were divided into three groups: (1) with filling of the defect with an ABC, (2) NFC-1-with filling of both the cortical plate and intramedullary space in the defect area, and (3) NFC-2-with filling of only the intramedullary space in the defect area. Histological and histomorphometric analysis was performed to assess the healing of the bone defect after 14, 30 and 60 days.
Complete closure of the cortical plate defect was detected in the NFC-2 group on Day 30 ( < 0.0001). Moreover, in both NFC groups on the 30th and 60th days, ongoing osteogenesis was observed, characterized by a large volume of newly formed circular pattern bone tissue in the intramedullary space.
СONCLUSION: This study demonstrated that the NFC-based composite, which is located below the level of the cortical plate, tamponing only the intramedullary space (NFC-2), improves bone tissue repair at the site of a bone defect of the cortical plate and has the potential of prolonged osteoinductivity.
Not applicable.
本研究旨在通过组织学和组织形态计量学评估，在体内评估和比较基于纳米原纤化纤维素（NFC）的复合材料与二水磷酸二钙和自体血凝块（ABC）对新骨组织形成的影响。
总共使用72只制造了股骨缺损（2毫米）的大鼠。将大鼠分为三组：（1）用ABC填充缺损；（2）NFC-1组，填充缺损区域的皮质板和髓腔；（3）NFC-2组，仅填充缺损区域的髓腔。在14、30和60天后进行组织学和组织形态计量学分析，以评估骨缺损的愈合情况。
在第30天，NFC-2组检测到皮质板缺损完全闭合（<0.0001）。此外，在第30天和第60天的两个NFC组中，均观察到持续的骨生成，其特征是髓腔内有大量新形成的圆形骨组织。
本研究表明，位于皮质板水平以下、仅填塞髓腔的基于NFC的复合材料（NFC-2）可改善皮质板骨缺损部位的骨组织修复，并具有延长骨诱导活性的潜力。
不适用。
