Behera Manisha, De Sachinandan, Ghorai Soma M
Department of Zoology, Hindu College, University of Delhi, Delhi, 110007, India.
Animal Biotechnology Centre, Animal Genomics Lab, National Dairy Research Institute (NDRI), Karnal, 132001, Haryana, India.
Probiotics Antimicrob Proteins. 2025 Apr;17(2):807-831. doi: 10.1007/s12602-024-10394-1. Epub 2024 Nov 7.
A major growing concern in the human and animal health sector is the emergence of antibiotic-resistant pathogenic bacteria due to the indiscriminate use of antibiotics. The exogenous application of bacteriophage endolysins causes abrupt lysis of the bacterial cell wall, which computes them as alternatives to antibiotics. Although naturally occurring endolysins may display limitations in solubility, lytic activity, and narrow lytic spectrum, novel strategies like developing chimeric endolysins and using endolysins in synergism with other antimicrobial agents are required to improve the lytic activity of natural endolysins. The modular structure of endolysins led to the development of novel chimeric endolysins via shuffling enzymatic and cell wall binding domains of different endolysins, using endolysins in a synergistic approach, and their applications in various in vitro and in vivo experiments and different applicable areas. This article aims to review the role of chimeric endolysins and their use in synergistic mode with other biofilm-reducing agents to control biofilm formation and deteriorating pre-formed biofilms in food, dairy, and medical industries. Promoting further development of phage technology and innovation in antibiotic therapy can achieve long-term sustainable development and economic returns.
由于抗生素的滥用，人类和动物健康领域一个日益严重的问题是出现了对抗生素具有抗性的病原菌。噬菌体溶菌酶的外源应用会导致细菌细胞壁突然裂解，这使其成为抗生素的替代品。尽管天然存在的溶菌酶在溶解性、裂解活性和裂解谱狭窄方面可能存在局限性，但需要开发嵌合溶菌酶以及将溶菌酶与其他抗菌剂协同使用等新策略来提高天然溶菌酶的裂解活性。溶菌酶的模块化结构通过改组不同溶菌酶的酶结构域和细胞壁结合结构域、以协同方式使用溶菌酶，以及它们在各种体外和体内实验及不同应用领域中的应用，促成了新型嵌合溶菌酶的开发。本文旨在综述嵌合溶菌酶的作用及其与其他生物膜减少剂协同模式下在食品、乳制品和医疗行业中控制生物膜形成和降解已形成生物膜方面的应用。促进噬菌体技术的进一步发展和抗生素治疗的创新可实现长期可持续发展和经济回报。
