Gong Mengxin, Li Mingyuan, Wang Jilian, Liu Jiaxuan, Meng Qing
Department of Biologic and Geographic Sciences, Kashi University, Kashi, Xinjiang, China.
Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, Kashi, Xinjiang, China.
Int Microbiol. 2025 Jun 20. doi: 10.1007/s10123-025-00687-y.
Enterobacteriaceae are significant opportunistic pathogens in the environment, impacting both biological and human health. The rise in antibiotic-resistant strains has compelled the exploration of bacteriophages as alternative biological control agents. In this study, we isolated a bacteriophage JgK1 specific to Escherichia coli from environmental samples. This bacteriophage can effectively lyse Escherichia coli jg1, exhibiting an optimal MOI of 0.01 and an average mutation rate of 1.96 × 10. One-step growth curve analysis revealed an incubation period of 25 min and a release amount of approximately 430.75 pfu/infection center. It can withstand high temperatures of 55 °C. Additionally, whole genome sequencing (WGS) confirmed a genome size of 74,059 bp, containing 107 open reading frames (ORF), with no virulence or resistance-related genes, nor lysogenic genes. Comparative genomic analysis indicated lower coverage compared to other known isolated bacteriophages, with the highest coverage being 72%. This study suggests that bacteriophage JgK1 has the potential to serve as an effective biological control antibacterial agent, offering a promising alternative to antibiotics for managing Escherichia coli infections.
肠杆菌科细菌是环境中重要的机会致病菌，对生物和人类健康均有影响。抗生素耐药菌株的增加促使人们探索噬菌体作为替代生物控制剂。在本研究中，我们从环境样本中分离出一种特异性针对大肠杆菌的噬菌体JgK1。这种噬菌体能够有效裂解大肠杆菌jg1，最佳感染复数为0.01，平均突变率为1.96×10⁻⁸。一步生长曲线分析显示潜伏期为25分钟，每个感染中心的释放量约为430.75个噬菌斑形成单位。它能耐受55℃的高温。此外，全基因组测序（WGS）证实基因组大小为74,059 bp，包含107个开放阅读框（ORF），没有毒力或抗性相关基因，也没有溶原性基因。比较基因组分析表明，与其他已知分离的噬菌体相比覆盖率较低，最高覆盖率为72%。本研究表明，噬菌体JgK1有潜力作为一种有效的生物控制抗菌剂，为管理大肠杆菌感染提供了一种有前景的抗生素替代方案。
