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基于热稳定抗原通过协同凝集对弯曲杆菌进行血清分型。

Serotyping of campylobacters by co-agglutination on the basis of heat-stable antigens.

作者信息

Fricker C R, Uradzinski J, Alemohammad M M, Park R W, Whelan C, Girdwood R W

出版信息

J Med Microbiol. 1986 Feb;21(1):83-6. doi: 10.1099/00222615-21-1-83.

Abstract

A new and simple method of serotyping campylobacters has been developed which utilises co-agglutination to detect the presence of heat-stable antigens. Campylobacters are heated at 75 degrees C for 30 min to destroy antigenic protein and allowed to react on a glass slide with staphylococci coated with antibody. Of 74 isolates, 67 gave the same result by co-agglutination and the previously described passive haemagglutination method. The co-agglutination technique may be used as a rapid screening test before serotyping by passive haemagglutination.

摘要

一种新的、简单的弯曲杆菌血清分型方法已经开发出来,该方法利用协同凝集来检测热稳定抗原的存在。将弯曲杆菌在75摄氏度下加热30分钟以破坏抗原性蛋白质,然后使其在载玻片上与包被有抗体的葡萄球菌发生反应。在74株分离株中,67株通过协同凝集和先前描述的被动血凝方法得到相同结果。协同凝集技术可在通过被动血凝进行血清分型之前用作快速筛选试验。

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