Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, 315211, China.
School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China.
Mikrochim Acta. 2024 Nov 8;191(12):732. doi: 10.1007/s00604-024-06821-3.
As one of the most harmful pathogenic bacteria in shrimp aquaculture, Vibrio parahaemolyticus often causes massive mortality in shrimp. Accurate and rapid detection of V. parahaemolyticus in shrimp farming is essential for avoiding huge economic losses caused by related diseases. In this study, we designed a dual-recognition platform for efficient identification and quantification of V. parahaemolyticus. First, the target bacterium was captured with magnetic beads functionalized by aptamers (Apt-MBs), and then, the broad-spectrum fluorescent probe FcMBL@CdSe-ZnS was used to detect the bacterium based on the interactions between fragment crystallizable mannose-binding lectin (FcMBL) and pathogenic bacteria. The proposed dual-recognition strategy centered around aptamers and FcMBL@CdSe-ZnS was applied to definite quantification of V. parahaemolyticus over a wide range of 10-10 CFU/mL with a limit of detection of 4 CFU/mL within 55 min. The feasibility was demonstrated by using the platform to detect V. parahaemolyticus from shrimp intestine, aquaculture water, and seawater.
作为虾类养殖中最具危害性的病原菌之一，副溶血性弧菌常导致虾类大量死亡。准确、快速地检测虾类养殖中的副溶血性弧菌对于避免相关疾病造成的巨大经济损失至关重要。在本研究中，我们设计了一种双识别平台，用于高效识别和定量检测副溶血性弧菌。首先，通过适配体功能化的磁性珠（Apt-MBs）捕获目标细菌，然后，基于片段结晶化甘露糖结合 lectin（FcMBL）与病原菌之间的相互作用，使用广谱荧光探针 FcMBL@CdSe-ZnS 检测细菌。该双识别策略以适配体和 FcMBL@CdSe-ZnS 为中心，可在 10-10 CFU/mL 的较宽范围内对副溶血性弧菌进行明确定量，检测限为 4 CFU/mL，检测时间为 55 分钟。该平台用于检测虾肠、养殖水和海水中的副溶血性弧菌，验证了其可行性。
