Department of Pharmacy, Xiaolan People's Hospital of Zhongshan, Zhongshan, 528415, Guangdong, PR China.
Pharmacology Laboratory, Zhongshan Hospital, Guangzhou University of Chinese Medicine, Zhongshan, 528401, Guangdong, PR China.
Sci Rep. 2024 Nov 8;14(1):27196. doi: 10.1038/s41598-024-78833-1.
As a traditional Chinese medicine formula used in clinical practice for an extended period, Suxin-Hugan-Fang (SXHGF) exhibits excellent anti-inflammatory properties. However, the efficacy of SXHGF in treating ulcerative colitis (UC) and its mechanism of action are still unclear. In this study, the therapeutic effects of SXHGF on UC were evaluated using network pharmacology and experimental validation, while also investigating its mechanism of action. By administering DSS to C57BL/6 mice to construct a mouse model of ulcerative colitis, the therapeutic effect of SXHGF on ulcerative colitis was evaluated based on weight loss percentage, disease activity index, colon length changes, and pathological conditions as indicators. The main chemical components of SXHGF were determined by LC-MS-QTOF method. The potential targets and mechanisms of action of SXHGF in the treatment of UC were inferred using bioinformatics methods, and further validated through ELISA, IHC, and Western blotting assays. The experimental results demonstrate that SXHGF can suppress oxidative stress and oxidative damage in the colon tissue of UC mice, and alleviate DSS-induced ulcerative colitis by inhibiting the JAK2/STAT3 and NFκB pathways.
作为一种在临床实践中长期使用的中药方剂，舒心护肝方（SXHGF）具有出色的抗炎特性。然而，SXHGF 治疗溃疡性结肠炎（UC）的疗效及其作用机制尚不清楚。在这项研究中，我们采用网络药理学和实验验证的方法评估了 SXHGF 治疗 UC 的疗效，并探讨了其作用机制。通过给予 C57BL/6 小鼠 DSS 来构建溃疡性结肠炎小鼠模型，我们以体重减轻百分比、疾病活动指数、结肠长度变化和病理状况等指标评估 SXHGF 对溃疡性结肠炎的治疗效果。采用 LC-MS-QTOF 方法测定 SXHGF 的主要化学成分。我们通过生物信息学方法推断了 SXHGF 治疗 UC 的潜在靶点和作用机制，并通过 ELISA、免疫组化和 Western blot 实验进行了进一步验证。实验结果表明，SXHGF 可抑制 UC 小鼠结肠组织中的氧化应激和氧化损伤，并通过抑制 JAK2/STAT3 和 NFκB 通路缓解 DSS 诱导的溃疡性结肠炎。
