Yang Xiaoyue, Gao Yuchao, Reyimu Muyesaier, Zhang Guoqiang, Wang Chunjuan, Yang Desong, Han Xiaoqiang
The Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Utilization, College of Agriculture, Shihezi University, Shihezi 832003, Xinjiang, China.
The Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Utilization, College of Agriculture, Shihezi University, Shihezi 832003, Xinjiang, China.
Int J Biol Macromol. 2024 Dec;282(Pt 5):137396. doi: 10.1016/j.ijbiomac.2024.137396. Epub 2024 Nov 7.
A novel polysaccharide, named as PFP1-1 (23 kDa), was isolated from the fruiting body of Pleurotus ferulae. Structural analysis revealed that PFP1-1 is primarily composed of mannose, galactose, glucose and fucose, with a molar ratio of 41.50:41.92:4.65:1.93. Infrared spectroscopy analysis showed the presence of characteristic absorption peaks associated with polysaccharides. Further analysis using gas chromatography-mass spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR) indicated that the polysaccharide mainly composed of → 6) -α-D-Galp- (1 →, → 2,6) -α-D-Galp- (1 → and a small amount of → 4) -α-D-Glcp- (1 →. The branched chain is mainly composed of β-D-Manp- (1 → and α-D-Glcp- (1 → connected at the O-2 position of the sugar residue → 2,6) -α-D-Galp- (1 →. PFP1-1 exhibited significant antifungal activity against Rhizoctonia solani and promoted cucumber plant growth. The mycelial growth inhibition rate of PFP1-1 against R. solani reached 70 %. In pot experiments, cucumber seedlings treated with PFP1-1 demonstrated resistance to R. solani infection and the incidence rate was significantly reduced to 22.92 %. PFP1-1 increased the root length and fresh weight of cucumber seedlings and enhanced the stress and disease resistance of plants by increasing the activities of superoxide dismutase, peroxidase and polyphenol oxidase. In conclusion, the present study provides a theoretical and experimental basis for the application of P. ferulae polysaccharide in promoting plant growth and controlling plant diseases.
从阿魏侧耳子实体中分离出一种名为PFP1-1（23 kDa）的新型多糖。结构分析表明，PFP1-1主要由甘露糖、半乳糖、葡萄糖和岩藻糖组成，摩尔比为41.50:41.92:4.65:1.93。红外光谱分析显示存在与多糖相关的特征吸收峰。使用气相色谱-质谱联用仪（GC-MS）和核磁共振（NMR）进一步分析表明，该多糖主要由→6)-α-D-半乳吡喃糖-(1→、→2,6)-α-D-半乳吡喃糖-(1→和少量的→4)-α-D-葡糖吡喃糖-(1→组成。支链主要由β-D-甘露吡喃糖-(1→和α-D-葡糖吡喃糖-(1→在糖残基→2,6)-α-D-半乳吡喃糖-(1→的O-2位连接而成。PFP1-1对茄丝核菌表现出显著的抗真菌活性，并促进黄瓜植株生长。PFP1-1对茄丝核菌的菌丝生长抑制率达到70%。在盆栽试验中，用PFP1-1处理的黄瓜幼苗对茄丝核菌感染表现出抗性，发病率显著降低至22.92%。PFP1-1增加了黄瓜幼苗的根长和鲜重，并通过提高超氧化物歧化酶、过氧化物酶和多酚氧化酶的活性增强了植物的抗逆性和抗病性。总之，本研究为阿魏多糖在促进植物生长和防治植物病害方面的应用提供了理论和实验依据。
