Khalafiyan Anis, Fadaie Mahmood, Khara Fatemeh, Zarrabi Ali, Moghadam Fariborz, Khanahmad Hossein, Cordani Marco, Boshtam Maryam
Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Drug Discov Today. 2024 Dec;29(12):104224. doi: 10.1016/j.drudis.2024.104224. Epub 2024 Nov 8.
Autophagy, the lysosome-driven breakdown of intracellular components, is pivotal in regulating eukaryotic cellular processes and maintaining homeostasis, making it physiologically important even under normal conditions. Cellular mechanisms involving autophagy include the response to nutrient deprivation, intracellular quality control, early development, and cell differentiation. Despite its established health significance, the role of autophagy in cancer and other diseases remains complex and not fully understood. A comprehensive understanding of autophagy is crucial to facilitate the development of novel therapies and drugs that can protect and improve human health. High-throughput technologies, such as single-cell RNA sequencing (scRNA-seq), have enabled researchers to study transcriptional landscapes at single-cell resolution, significantly advancing our knowledge of autophagy pathways across diverse physiological and pathological contexts. This review discusses the latest advances in scRNA-seq for autophagy research and highlights its potential in the molecular characterization of various diseases.
自噬是由溶酶体驱动的细胞内成分分解过程，在调节真核细胞过程和维持体内平衡方面起着关键作用，这使其即使在正常条件下也具有重要的生理意义。涉及自噬的细胞机制包括对营养剥夺的反应、细胞内质量控制、早期发育和细胞分化。尽管自噬已被证实对健康具有重要意义，但其在癌症和其他疾病中的作用仍然复杂且尚未完全明确。全面了解自噬对于推动能够保护和改善人类健康的新型疗法和药物的开发至关重要。高通量技术，如单细胞RNA测序（scRNA-seq），使研究人员能够在单细胞分辨率下研究转录图谱，显著推进了我们对不同生理和病理背景下自噬途径的认识。本综述讨论了scRNA-seq在自噬研究中的最新进展，并强调了其在各种疾病分子特征分析中的潜力。
