追踪空气中的新冠病毒RNA：来自一个医务室新冠疫情的经验教训。
Tracking SARS-CoV-2 RNA in the air: Lessons from a COVID-19 outbreak in an infirmary unit.
作者信息
Wong Shuk-Ching, Chen Jonathan Hon-Kwan, Chau Pui-Hing, Tam Wai-On, Lam Germaine Kit-Ming, Yuen Lithia Lai-Ha, Chan Wan-Mui, Chu Allen Wing-Ho, Ip Jonathan Daniel, Tsoi Hoi-Wah, So Simon Yung-Chun, Chiu Kelvin Hei-Yeung, Yuen Kwok-Yung, To Kelvin Kai-Wang, Cheng Vincent Chi-Chung
机构信息
Infection Control Team, Queen Mary Hospital, Hong Kong West Cluster, Pokfulam, Hong Kong Special Administrative Region of China; Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region of China; School of Nursing, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region of China.
Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region of China; Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region of China.
出版信息
Am J Infect Control. 2025 Mar;53(3):348-356. doi: 10.1016/j.ajic.2024.10.035. Epub 2024 Nov 8.
BACKGROUND
The duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreaks remain uncertain. This study evaluates the impact of mobile modular high-efficiency particulate air filter units (MMHUs) on SARS-CoV-2 air dispersal.
METHODS
We investigated a nosocomial COVID-19 outbreak in an infirmary unit. The viral load (VL) of SARS-CoV-2 in air samples was correlated with the VL in nasopharyngeal swabs with or without MMHU. These samples underwent whole-genome sequencing and phylogenetic analysis.
RESULTS
Upon outbreak declaration (August 2, 2024, day 0), 44 (69.8%) of 63 patients acquired COVID-19 in Ward 2B (19 male) and 2C (25 female) by day 4. The VL of SARS-CoV-2 remained detectable in air until day 11 (2B) and day 20 (2C). The VL of air samples was significantly correlated with the VL in nasopharyngeal swabs collected on days 5, 7, 10, and 13 in 2C (r = -0.975, P = .004). Using MMHU, the mean daily ratio of SARS-CoV-2 RNA (copies/1,000 L of air/patient) in 2B was 5 times lower than in 2C from days 5 to 10. Whole-genome sequencing revealed all 41 tested strains belonged to the Omicron variant, KP.3.1.1, phylogenetically related to the prevailing community strains.
CONCLUSIONS
Using MMHU mitigates the duration and magnitude of SARS-CoV-2 air dispersal during nosocomial outbreak.
背景
在医院爆发期间，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）空气传播的持续时间和程度仍不确定。本研究评估了移动模块化高效空气过滤器装置（MMHU）对SARS-CoV-2空气传播的影响。
方法
我们调查了一个医务室单元内的医院新冠肺炎疫情爆发情况。对有或没有MMHU的情况下，空气样本中SARS-CoV-2的病毒载量（VL）与鼻咽拭子中的VL进行相关性分析。这些样本进行了全基因组测序和系统发育分析。
结果
在宣布爆发疫情时（2024年8月2日，第0天），到第4天，63名患者中有44名（69.8%）在2B病房（19名男性）和2C病房（25名女性）感染了新冠肺炎。SARS-CoV-2的VL在空气中直到第11天（2B病房）和第20天（2C病房）仍可检测到。2C病房空气样本的VL与第5、7、10和13天采集的鼻咽拭子中的VL显著相关（r = -0.975，P = 0.004）。使用MMHU后，从第5天到第10天，2B病房SARS-CoV-2 RNA（每1000升空气/患者的拷贝数）的平均每日比率比2C病房低5倍。全基因组测序显示，所有41株测试菌株均属于奥密克戎变种KP.3.1.1，在系统发育上与流行的社区菌株相关。
结论
在医院爆发期间，使用MMHU可减轻SARS-CoV-2空气传播的持续时间和程度。
Zotero 插件