Department of Plastic and Reconstructive Surgery, Chang-Gung Memorial Hospital, Chang-Gung University and Medical College, Taoyuan, Taiwan.
Department of Plastic and Reconstructive Surgery and Craniofacial Research Center, Chang Gung Memorial Hospital at Linkou, Taoyuan, 33305, Taiwan.
Int J Nanomedicine. 2024 Nov 7;19:11463-11477. doi: 10.2147/IJN.S492244. eCollection 2024.
Deep, second- and third-degree burn injuries may lead to irreversible damage to the traumatized tissue and to coagulation or thrombosis of the microvessels, further compromising wound healing. Engineered, morphologically gradient drug-eluting nanofiber dressings promote wound healing by mimicking tissue structure and providing sustained drug delivery, which is particularly beneficial for wound management.
This study exploited a resorbable, radially aligned nanofiber dressing that provides the sustained gradient release of metformin at the wound site using a pin-ring electrospinning technique and a differential membrane-thickness approach.
The experimental results suggested that the electrospun nanofibrous dressings exhibited uniform and radially oriented fiber distributions. In vitro, these dressings offered an extended release of metformin for 30 d. The incorporation of water-soluble metformin significantly enhanced the hydrophilicity of the nanofiber membranes. Moreover, the in vivo burn-wound-healing model of rats showed that the radially aligned gradient metformin-eluting poly(lactic-co-glycolic acid) (PLGA) nanofibers exhibited significantly superior healing capability compared to the pristine PLGA, metformin-eluting, and control dressings. Histological images showed that the mesh/nanofibers produced no adverse effects.
The findings in this study emphasize the potential of resorbable, radially aligned nanofiber dressings as advanced wound care solutions, offering broad applicability and meaningful clinical impact.
深度、二度和三度烧伤可能导致受伤组织的不可逆损伤，并导致微血管的凝血或血栓形成，进一步影响伤口愈合。工程化的形态梯度药物洗脱纳米纤维敷料通过模拟组织结构和提供持续的药物输送来促进伤口愈合，这对伤口管理尤其有益。
本研究利用可吸收的、径向排列的纳米纤维敷料，通过针环静电纺丝技术和差分膜厚方法，在伤口部位提供持续的梯度释放二甲双胍。
实验结果表明，电纺纳米纤维敷料具有均匀的、径向取向的纤维分布。在体外，这些敷料可提供长达 30 天的二甲双胍延长释放。水溶性二甲双胍的掺入显著提高了纳米纤维膜的亲水性。此外，大鼠烧伤伤口愈合模型表明，与原始的聚乳酸-共-羟基乙酸（PLGA）、二甲双胍洗脱和对照敷料相比，径向排列的梯度二甲双胍洗脱 PLGA 纳米纤维表现出显著优越的愈合能力。组织学图像显示，网格/纳米纤维没有产生任何不良影响。
本研究的结果强调了可吸收的、径向排列的纳米纤维敷料作为先进的伤口护理解决方案的潜力，具有广泛的适用性和有意义的临床影响。
