de Alexandria Francisco Eugênio Deusdará, Silva Naldiana Cerqueira, Assis Livia, Filho Antônio Luiz Martins Maia, Kido Hueliton Wilian, Tarocco Juliana Carolina, Ferreira Rui Seabra, Barraviera Benedito, Parizotto Nivaldo Antônio, Silva José Figueiredo, Neto Messias Augusto das Neves, Tim Carla Roberta
Scientific and Technological Institute, Universidade Brasil, Sao Paulo (SP), Brazil.
Center for Research in Biotechnology and Biodiversity, Universidade Estadual do Piauí, Teresina (PI), Brazil.
Lasers Med Sci. 2024 Nov 15;39(1):279. doi: 10.1007/s10103-024-04229-2.
Diabetes mellitus is characterized by elevated blood glucose levels causing sometimes impairment of the body's ability to repair itself. Promising treatments for tissue repair have included photobiomodulation therapy and heterologous fibrin biopolymer (HFB). This study aimed to evaluate the impact of photobiomodulation therapy by LED, both as a standalone treatment and in conjunction with heterologous fibrin biopolymer in treatment of skin lesions of diabetic rats. Diabetes was induced using alloxan. Full-thickness skin wounds were induced on the backs of 56 Wistar rats, which were randomly allocated into four groups: control group, heterologous fibrin biopolymer group, photobiomodulation therapy by LED group, and photobiomodulation therapy by LED combined with heterologous fibrin biopolymer group. The treatments spanned two experimental periods, lasting 7 and 14 days. Notably, the HFB group exhibited results similar to those of the LED group concerning wound regression, while demonstrating superior resistance to healing. Interestingly, the LED + HFB group showed greater skin damage at 7 days, but an improved repair process at 14 days compared to the control group. The findings indicate that combining photobiomodulation by LED with HFB did not enhance wound healing in diabetic rats beyond the effects of each treatment alone. Both treatments were effective individually, with HFB showing particular strength in promoting collagen maturation and improving tissue biomechanical properties. This study contributes to the ongoing body of research on skin repair with this innovative HFB. Future clinical trials will be essential to validate this proposition.
糖尿病的特征是血糖水平升高，有时会损害身体的自我修复能力。有前景的组织修复治疗方法包括光生物调节疗法和异源纤维蛋白生物聚合物（HFB）。本研究旨在评估发光二极管（LED）光生物调节疗法单独使用以及与异源纤维蛋白生物聚合物联合使用对糖尿病大鼠皮肤损伤治疗的影响。使用四氧嘧啶诱导糖尿病。在56只Wistar大鼠的背部造成全层皮肤伤口，将其随机分为四组：对照组、异源纤维蛋白生物聚合物组、LED光生物调节疗法组和LED光生物调节疗法联合异源纤维蛋白生物聚合物组。治疗跨越两个实验期，持续7天和14天。值得注意的是，HFB组在伤口消退方面表现出与LED组相似的结果，同时显示出更强的愈合抵抗力。有趣的是，LED + HFB组在第7天时皮肤损伤更大，但与对照组相比，在第14天时修复过程有所改善。研究结果表明，将LED光生物调节与HFB联合使用并不会比单独使用每种治疗方法更能促进糖尿病大鼠的伤口愈合。两种治疗方法单独使用均有效，HFB在促进胶原蛋白成熟和改善组织生物力学性能方面表现出特别的优势。本研究为关于这种创新型HFB的皮肤修复研究不断发展的体系做出了贡献。未来的临床试验对于验证这一观点至关重要。
