Jeong Sumin, Han Jong Won, Kim Yeseul, Bak Eunjeong, Ma Kyung Ho, Lee Jeong Hoon, Jung Jin Tae, Park Inkyu
Department of Biology and Chemistry, Changwon National University, Changwon, 51140, South Korea.
Research Management Division, Rural Development Administration, Jeonju, 54875, South Korea.
Genes Genomics. 2025 Jan;47(1):99-112. doi: 10.1007/s13258-024-01573-z. Epub 2024 Nov 14.
In Korea, only Polygala tenuifolia is registered as Polygalae Radix in the pharmacopoeia, while in China, both P. tenuifolia and P. sibirica are used equally. Accurate identification of herbal medicines is crucial for their safety and efficacy, but commercial products are typically sold in dried form, making morphological distinction difficult. Therefore, a quick and accurate method to distinguish P. tenuifolia is necessary for proper utilization of medicinal herb.
We aimed to identify specific molecular markers for P. tenuifolia to avoid confusion regarding its pharmacological efficacy and to evaluate the classification of Polygala using plastid phylogenetic data.
We analyzed the sequences of three species distributed in Korea, P. tenuifolia, P. japonica, and P. sibirica, and assembled their chloroplast genome sequences. Comparative analysis revealed regions of local divergence, and six molecular markers were developed from these hotspots. Additionally, a phylogenetic tree was constructed to determine the phylogenetic positions of the three Polygala species.
The marker successfully identified the three Polygala species, and all commercial products and breeding lines tested were confirmed to be P. tenuifolia and recognized as authentic. Phylogenetic analysis revealed that P. tenuifolia forms a distinct cluster from P. sibirica and P. japonica.
We determined the chloroplast genomes of the three Polygala species and performed phylogenetic tree analysis and marker development. Indel markers were developed to identify the critical herbal species, P. tenuifolia. This comprehensive study of the Polygala chloroplast genome provides useful information for P. tenuifolia identification.
在韩国，药典中仅远志被登记为远志根，而在中国，细叶远志和西伯利亚远志均被同等使用。准确鉴定草药对于其安全性和有效性至关重要，但商业产品通常以干燥形式出售，这使得形态学区分变得困难。因此，为了合理利用药用植物，需要一种快速准确的方法来区分细叶远志。
我们旨在鉴定细叶远志的特定分子标记，以避免其药理功效方面的混淆，并利用质体系统发育数据评估远志属的分类。
我们分析了分布在韩国的三种远志属植物，即细叶远志、瓜子金和西伯利亚远志的序列，并组装了它们的叶绿体基因组序列。比较分析揭示了局部差异区域，并从这些热点区域开发了六个分子标记。此外，构建了系统发育树以确定三种远志属植物的系统发育位置。
该标记成功鉴定了三种远志属植物，所有测试的商业产品和育种系均被确认为细叶远志并被认定为正品。系统发育分析表明，细叶远志与西伯利亚远志和瓜子金形成了一个独特的聚类。
我们确定了三种远志属植物的叶绿体基因组，并进行了系统发育树分析和标记开发。开发了插入缺失标记以鉴定关键草药物种细叶远志。这项对远志属叶绿体基因组的全面研究为细叶远志的鉴定提供了有用信息。
