使用 MitoPlates™ 和突触神经小体进行突触线粒体的代谢表型分析。
Metabolic Phenotyping of Synaptic Mitochondria Using MitoPlates™ and Synaptoneurosomes.
机构信息
Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
出版信息
Methods Mol Biol. 2025;2878:67-74. doi: 10.1007/978-1-0716-4264-1_4.
Mitochondrial functional assays using MitoPlates™ S-1 allow us to characterize mitochondria in terms of substrate metabolism. MitoPlates™ are 96-well microplates pre-coated with a diverse set of substrates. The electron flow from NADH and FADH producing mitochondrial substrates is measured based on the reduction of redox dye, that acts as a terminal electron acceptor. Here, we describe the application of MitoPlates™ to characterize the metabolism of synaptic mitochondria enclosed in isolated pre- and postsynaptic terminals (synaptoneurosomes).
使用 MitoPlates™ S-1 进行线粒体功能测定可以让我们从底物代谢的角度来描述线粒体。MitoPlates™ 是一种 96 孔微孔板，预先涂有多种底物。来自产生线粒体底物的 NADH 和 FADH 的电子流是根据氧化还原染料的还原来测量的，该染料作为末端电子受体。在这里，我们描述了 MitoPlates™ 在描述包含在分离的突触前和突触后末端（突触小体）中的突触线粒体代谢中的应用。
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