整合转录组学和蛋白质组学数据：IL-27B作为脓毒症性心肌病发展中的关键蛋白——一项回顾性研究

Integrating Transcriptomic and Proteomic Data: IL-27B as a Key Protein in the Development of Septic Cardiomyopathy-A Retrospective Study.

作者信息

Mao Yifeng, Chen Qingqing, Jiang Yongpo, Zhang Xijiang, Si Qin, Xu Panpan, Zhang Zhongheng, Zheng Cheng, Lin Ronghai

机构信息

Department of Critical Care Medicine, Municipal Hospital Affiliated to Taizhou University, Taizhou, ZheJiang Province, China.

Department of Critical Care Medicine, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, Zhejiang Province, China.

出版信息

Immun Inflamm Dis. 2025 May;13(5):e70207. doi: 10.1002/iid3.70207.

DOI:10.1002/iid3.70207

PMID:40396598

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12093348/

Abstract

BACKGROUND

Septic cardiomyopathy (SCM) is a potentially fatal complication of sepsis. In this study, transcriptomic and proteomic analyzes of serum samples from sepsis patients were conducted to uncover the underlying pathological mechanisms and identify potential therapeutic targets for SCM.

METHODS

This retrospective, dual-center study investigated the progression of sepsis to SCM in patients admitted to intensive care units. A total of 50 patients were enrolled and divided into two groups: sepsis with cardiomyopathy (25 cases) and sepsis without cardiomyopathy (25 cases). Co-expression network analysis was employed to elucidate the biological significance of differentially expressed proteins. By integrating proteomic and transcriptomic data, molecular networks were constructed to visualize interactions among key molecules, aiming to enhance data interpretation and support the study's findings.

RESULTS

Proteomic analysis identified 216 differentially expressed proteins (Fold change > 1.5, p-value < 0.05) between the two groups. Transcriptomic analysis revealed two proteins, including Interleukin-27 subunit beta (IL-27B) and carbonic anhydrase, co-downregulated in patients with septic cardiomyopathy. IL-27B was associated with the immune response, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated its involvement in the cytokine-cytokine receptor interaction signaling pathway.

CONCLUSION

Comprehensive integrated transcriptomic and proteomic analyzes identified significant changes in protein expression associated with SCM, primarily associated with inflammation-related pathways and amino acid metabolism. These findings provide new insights into the pathological mechanisms of SCM and highlight potential therapeutic targets for its treatment.

TRIAL REGISTRATION

The Clinical Research Ethics Committee of Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University approved this study, and written informed consent was given by all patients or their legal representatives. (NO.K20201110).

摘要

背景

脓毒症性心肌病（SCM）是脓毒症一种潜在的致命并发症。在本研究中，对脓毒症患者的血清样本进行了转录组学和蛋白质组学分析，以揭示潜在的病理机制并确定SCM的潜在治疗靶点。

方法

这项回顾性、双中心研究调查了重症监护病房收治患者中脓毒症进展为SCM的情况。共纳入50例患者，分为两组：脓毒症合并心肌病组（25例）和脓毒症无心肌病组（25例）。采用共表达网络分析来阐明差异表达蛋白的生物学意义。通过整合蛋白质组学和转录组学数据，构建分子网络以可视化关键分子之间的相互作用，旨在增强数据解读并支持研究结果。

结果

蛋白质组学分析确定两组之间有216种差异表达蛋白（倍数变化>1.5，p值<0.05）。转录组学分析显示两种蛋白，包括白细胞介素27β亚基（IL-27B）和碳酸酐酶，在脓毒症性心肌病患者中共同下调。IL-27B与免疫反应相关，京都基因与基因组百科全书（KEGG）通路富集分析表明其参与细胞因子-细胞因子受体相互作用信号通路。