Shuo Tang, Haoting Niu, Yuqing Wang, Liuyun Jiang, Xiang Hu
National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, Key Laboratory of Light Energy Conversion Materials of Hunan Province College, Hunan Normal University, Changsha 410081, PR China.
State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, PR China.
Int J Biol Macromol. 2024 Dec;283(Pt 3):137618. doi: 10.1016/j.ijbiomac.2024.137618. Epub 2024 Nov 15.
To improve chitosan hemostasis, carboxylated sisal fiber and kaolin were introduced to obtain carboxylated sisal fiber/chitosan/kaolin (SF/CS/K) composite sponges (the weight ratio of 3: 3:4, 4:4:2, 5:5:0) by freeze-drying method. The results showed that the ionic cross-linking of the carboxylated sisal fiber with chitosan and kaolin-loading endowed the composite sponges with not only oriented groove porous structure, high mechanical strength, porosity, water absorption and compress recovery, but also suitable biodegradation, good cytocompatibility, hemocompatibility, protein adsorption, antibacterial activity. Especially, compared with commercial gelatin hemostatic sponges, the composite sponge of SF/CS/K displayed better coagulation ability and hemostatic effect, and animal experiments further demonstrated that the bleeding amount and hemostatic time of SF/CS/K were greatly reduced in rat hemostatic models of tail amputation, femoral vein trauma and liver injury, owing to the synergistic hemostatic effect of chitosan, kaolin and sisal fiber, as well as the groove porous structure, which endowed them with strong adhesion for red blood cells. Conclusively, SF/CS/K composite sponge had the best hemostatic effect because of the most appropriate component ratio, which is a novel promising natural hemostatic sponge for effective and rapid hemostasis in deep massive bleeding sites.
为改善壳聚糖的止血性能，引入羧化剑麻纤维和高岭土，通过冷冻干燥法制备羧化剑麻纤维/壳聚糖/高岭土（SF/CS/K）复合海绵（重量比为3:3:4、4:4:2、5:5:0）。结果表明，羧化剑麻纤维与壳聚糖的离子交联以及负载高岭土赋予复合海绵不仅具有定向沟槽多孔结构、高机械强度、孔隙率、吸水性和压缩回复性，还具有合适的生物降解性、良好的细胞相容性、血液相容性、蛋白质吸附性和抗菌活性。特别是，与市售明胶止血海绵相比，SF/CS/K复合海绵表现出更好的凝血能力和止血效果，动物实验进一步证明，在大鼠断尾、股静脉创伤和肝损伤止血模型中，由于壳聚糖、高岭土和剑麻纤维的协同止血作用以及沟槽多孔结构赋予它们对红细胞的强粘附力，SF/CS/K的出血量和止血时间大大减少。总之，SF/CS/K复合海绵因其最适宜的组分比例而具有最佳止血效果，是一种新型且有前景的天然止血海绵，可在深部大量出血部位实现有效快速止血。
