磷脂酰胆碱主体双层膜中磷脂酰丝氨酸和磷脂酰乙醇胺的固有pKa值。
The intrinsic pKa values for phosphatidylserine and phosphatidylethanolamine in phosphatidylcholine host bilayers.
作者信息
Tsui F C, Ojcius D M, Hubbell W L
出版信息
Biophys J. 1986 Feb;49(2):459-68. doi: 10.1016/S0006-3495(86)83655-4.
Abstract
Potentiometric titrations and surface potential measurements have been used to determine the intrinsic pKa values of both the carboxyl and amino groups of phosphatidylserine (PS) in mixed vesicles of PS and phosphatidylcholine (PC), and also of the amino group of phosphatidylethanolamine (PE) in mixed PE-PC vesicles. The pKa of the carboxyl group of PS in liposomes with different PS/PC lipid ratios measured by the two different methods is 3.6 +/- 0.1, and the pKa of its amino group is 9.8 +/- 0.1. The pKa of the amino group of PE in PE-PC vesicles, determined solely by surface potential measurements, is 9.6 +/- 0.1. These pKa values are independent of the aqueous phase ionic strength and of the effect of the liposome's surface potential due to the presence of these partially charged lipids.
摘要
电位滴定法和表面电位测量法已被用于测定磷脂酰丝氨酸（PS）与磷脂酰胆碱（PC）混合囊泡中PS羧基和氨基的固有pKa值，以及磷脂酰乙醇胺（PE）与PC混合囊泡中PE氨基的固有pKa值。通过两种不同方法测量的不同PS/PC脂质比脂质体中PS羧基的pKa为3.6±0.1，其氨基的pKa为9.8±0.1。仅通过表面电位测量确定的PE-PC囊泡中PE氨基的pKa为9.6±0.1。这些pKa值与水相离子强度以及由于这些部分带电脂质的存在而产生的脂质体表面电位的影响无关。
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