Qin Kaiqi, Huang Xinyue, Wang Shengfeng, Liang Jiachen, Fan Zengjie
Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
Adv Healthc Mater. 2025 Jan;14(2):e2403840. doi: 10.1002/adhm.202403840. Epub 2024 Nov 17.
Repairing osteochondral (OC) defect presents a significant challenge due to the intricate structural requirements and the unpredictable differentiation pathways of bone marrow mesenchymal stem cells (BMSCs). To address this challenge, a novel biomimetic OC hydrogel scaffold is developed that features a structure of soft and hard components. This scaffold incorporates bilayer metal-organic frameworks (MOFs), specifically ZIF-67 in the upper layer and ZIF-8 in the lower layer, achieved through an in situ printing process. This configuration enables the spatial and temporal modulation of BMSC differentiation by controlling the release of Co⁺ and Zn⁺. The results demonstrate that the bilayer MOF hydrogels significantly outperform hydrogels that either lack MOFs or contain a single type of MOF in enhancing repair outcomes in rabbit models of knee OC defects. The improved regenerative efficacy is attributed to the distinct chondrogenic and osteogenic differentiation cues provided by the bilayer MOFs, effectively guiding BMSCs toward enhanced tissue regeneration. This customizable biomimetic OC hydrogel scaffold not only opens new avenues for innovative therapeutic strategies but also holds great promise for widespread clinical applications.
修复骨软骨（OC）缺损是一项重大挑战，因为骨髓间充质干细胞（BMSCs）的结构要求复杂且分化途径不可预测。为应对这一挑战，开发了一种新型的仿生OC水凝胶支架，其具有软硬成分结构。该支架通过原位打印工艺结合了双层金属有机框架（MOFs），具体为上层的ZIF-67和下层的ZIF-8。这种结构通过控制Co⁺和Zn⁺的释放实现对BMSC分化的时空调节。结果表明，在兔膝关节OC缺损模型中，双层MOF水凝胶在增强修复效果方面显著优于缺乏MOFs或含有单一类型MOF的水凝胶。再生效果的改善归因于双层MOFs提供的独特软骨生成和成骨分化线索，有效地引导BMSCs促进组织再生。这种可定制的仿生OC水凝胶支架不仅为创新治疗策略开辟了新途径，也为广泛的临床应用带来了巨大希望。
