El-Zairy Amany H, Mohamed Hussein S, Ahmed Shimaa A, Ahmed Sayed A, Okla Mohammad K, El-Adl Khaled, AbdElgawad Hamada, Hozzein Wael N
Chemistry of medicinal and aromatic plants department, Research Institute of Medicinal and Aromatic plants (RIMAP), Beni-Suef University, Beni-Suef, 62511, Egypt.
Chemistry department, Faculty of Science, Beni-Suef University, Beni-Suef, 62511, Egypt.
Heliyon. 2024 Oct 25;10(21):e39612. doi: 10.1016/j.heliyon.2024.e39612. eCollection 2024 Nov 15.
Desert plants possess untapped potential for medicinal applications due to their rich phytochemical profiles. However, they need to be more explored. Thus, this study integrates advanced analytical, biochemical, and molecular techniques to investigate the phytochemical composition and biological activities (antimicrobial and antioxidant) of four desert plants (, and , collected from Wadi Sannor, Beni-Suef Governorate, Egypt, in March 2021. The volatile chemicals in the 70 % ethanol extracts of the selected plants were also analyzed using GC-MS. The extract exhibited strong antioxidant properties, as demonstrated by its FRAP (Ferric reducing ability of plasma) values, anti-lipid peroxidation, superoxide anion scavenging activity, and DPPH scavenging activity. Additionally, plants extracts showed high antimicrobial activities against seven pathogens, including three Gram-negative bacteria (, , ) and four Gram-positive bacteria (Staphylococcus saprophyticus, Staphylococcus epidermidis, , Streptococcus salivarius). Lastly, molecular docking was conducted for cis-vaccenic acid, (E)-9-octadecenoic acid, the cyclohepta[b]furan-2-one scaffold, and URS-20(30)-en-3-ol against both the thymidylate kinase enzyme and the active sites of DNA gyrase. The results from the molecular docking studies showed a strong correlation with the biological data. Moreover, these compounds exhibited good, proposed absorption, distribution, metabolism, and excretion-toxicity (ADMET) profiles. Our study highlights the potential of P. tomentosa, Z. coccineum, P. undulata, and O. baccatus for future medical applications and the development of new pharmaceuticals derived from desert flora.
由于其丰富的植物化学特征，沙漠植物在药用应用方面具有未被开发的潜力。然而，它们需要得到更多的探索。因此，本研究整合了先进的分析、生化和分子技术，以研究2021年3月从埃及贝尼苏韦夫省瓦迪桑诺采集的四种沙漠植物（绒毛番荔枝、朱红合欢、波叶金合欢和浆果仙人掌）的植物化学成分和生物活性（抗菌和抗氧化）。还使用气相色谱-质谱联用仪分析了所选植物70%乙醇提取物中的挥发性化学物质。提取物表现出很强的抗氧化性能，其血浆铁还原能力（FRAP）值、抗脂质过氧化、超氧阴离子清除活性和二苯基苦味酰基自由基（DPPH）清除活性都证明了这一点。此外，植物提取物对七种病原体表现出高抗菌活性，包括三种革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌）和四种革兰氏阳性菌（腐生葡萄球菌、表皮葡萄球菌、金黄色葡萄球菌、唾液链球菌）。最后，对顺式vaccenic酸、（E）-9-十八碳烯酸、环庚[b]呋喃-2-酮支架和URS-20（30）-烯-3-醇针对胸苷酸激酶和DNA促旋酶的活性位点进行了分子对接。分子对接研究的结果与生物学数据显示出很强的相关性。此外，这些化合物表现出良好的、推测的吸收、分布、代谢和排泄-毒性（ADMET）特征。我们的研究突出了绒毛番荔枝、朱红合欢、波叶金合欢和浆果仙人掌在未来医学应用以及开发源自沙漠植物群的新药物方面的潜力。
