Determination of Therapeutic and Safety Effects of Extract in Induced Inflammation in Rats.

BACKGROUND

is a facultative plant with many medicinal applications. This study examined the anti-inflammatory activity of () in an arthritis animal model.

MATERIALS AND METHODS

Seventy-Six Wistar Albino rats of either sex randomly divided into six groups (12/each). The inflammation model was done using Complete Freund's Adjuvant in albino rats. The anti-inflammatory activities of the extract were estimated at different dose levels (15.6, 31, and 60 mg/kg) as well as upon using methotrexate (MTX) as a standard drug (0.3 mg/kg). Paw volume and arthritis index scores have been tested in all examined animals' treatments. Histological examination of joints was also performed. Flow cytometric studies were done to isolated osteoclasts. Cytokines assay as well as biochemical testing was done in the examined samples. studies reported an IC of 15.6 g/ml for extract in lipoxygenase inhibition assay (L.O.X.). Moreover, it could be noticed that isorhamnetin-3-O-glucoside, tribuloside, and 7-acetoxy-4-methyl coumarin were the most common compounds in extract separated using L.C.-ESI-TOF-M.S. (liquid chromatography-electrospray ionization ion-trap time-of-flight mass spectrometry). Microscopic examinations of synovial tissue and hind limb muscles revealed the effect of different doses of extract on restoring chondrocytes and muscles structures. Osteoclast size and apoptotic rate examinations revealed the protective effect of extract on osteoclast. The results upon induction of animals and upon treatment using of MTX significantly increased apoptotic rate of osteoclast compared to control, while using of 15.6 g/ml. for extract lead to recover regular apoptotic rate demonstrating the protective effect of the extract. extract regulated the secretion of proinflammatory and anti-inflammatory cytokines. Biochemical tests indicated the safety of extract on kidney and liver functions. extract has efficient and safe anti-inflammatory potential in an induced rat model.