Baron M, Eyzaguirre C
Am J Physiol. 1977 Jul;233(1):C35-46. doi: 10.1152/ajpcell.1977.233.1.C35.
Excised cat carotid bodies and nerves were placed in a Lucite chamber through which saline flowed at different temperatures. Action potentials were recorded with a suction electrode. Glomus cells were impaled with microelectrodes and identified by ejecting Procion navy blue from the micropipette. Membrane potentials (MP) averaged 20 mV and input resistances (R0), 40 Momega. Cooling induced depolarization and decreased R0. This effect had a reversal potential of about -6 mV. [K+]0 did not affect MP, R0, or cooling effects. Lack of sodium increased MP, R0, and depressed cooling effects. Low chloride had opposite effects. Ca2+ and Mg2+ influenced MP and R0 but had little effects on temperature actions. Ouabain decreased MP and R0. Results indicate that glomus cells are exquisitely sensitive to temperature changes; their MP and R0 increase with high temperatures and decrease when temperature is lowered. These effects are mostly dependent on the external concentration of Cl- and Na+. Glomus cells may be the site of the intense metabolism of this tissue and thus contribute to the generation of chemosensory impulses.
将切除的猫颈动脉体和神经置于一个有机玻璃小室中，不同温度的生理盐水从中流过。用吸力电极记录动作电位。用微电极刺入球细胞，并通过从微量移液器中喷出普施安海军蓝来识别。膜电位（MP）平均为20mV，输入电阻（R0）为40MΩ。冷却引起去极化并降低R0。这种效应的反转电位约为 -6mV。细胞外钾离子浓度（[K+]0）不影响MP、R0或冷却效应。缺乏钠离子会增加MP、R0并抑制冷却效应。低氯离子浓度则产生相反的效果。钙离子和镁离子影响MP和R0，但对温度效应影响很小。哇巴因降低MP和R0。结果表明，球细胞对温度变化极为敏感；它们的MP和R0在高温时增加，温度降低时则降低。这些效应主要取决于细胞外氯离子和钠离子的浓度。球细胞可能是该组织强烈代谢的部位，因此有助于化学感觉冲动的产生。
