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的营养缺陷型（）突变体生长显著延迟，但生物钟正常。

The auxotrophic () mutant of has significantly delayed growth but a normal circadian clock.

作者信息

Wang Ziyan, Lindgren Kristin M, Loros Jennifer J, Dunlap Jay C

机构信息

Geisel School of Medicine at Dartmouth, Department of Molecular and Systems Biology, Hanover, NH, USA.

Geisel School of Medicine at Dartmouth, Department of Biochemistry and Cell Biology, Hanover, NH, USA.

出版信息

Fungal Genet Rep. 2024;68. doi: 10.4148/1941-4765.2185.

DOI:10.4148/1941-4765.2185
PMID:39555235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11565437/
Abstract

Some cell biological studies of have been limited by the rapid rates of hyphal growth and fusion. In this study, we investigated the causative mutation in the standard C24 allele of (FGSC #9) and assayed the growth and circadian phenotype of the strain under different nutritional conditions. We show that the strain can be maintained as metabolically active single cells for 2 days before its growth advances into branched mycelia. This culturing system offers the potential to advance subcellular dynamic research and to facilitate greater understanding of in the early developmental stages.

摘要

一些关于[具体研究对象]的细胞生物学研究受到菌丝快速生长和融合速度的限制。在本研究中，我们调查了[具体研究对象]标准C24等位基因（FGSC #9）中的致病突变，并检测了该[具体研究对象]菌株在不同营养条件下的生长和昼夜节律表型。我们发现，该[具体研究对象]菌株在生长进入分支菌丝体之前，可以作为代谢活跃的单细胞维持2天。这种培养系统为推进亚细胞动态研究以及促进对[具体研究对象]早期发育阶段的更深入理解提供了潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/abd5eed7d9ff/nihms-2031133-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/74fb68167525/nihms-2031133-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/dc11fd9bc90a/nihms-2031133-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/626b97a36ba2/nihms-2031133-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/abd5eed7d9ff/nihms-2031133-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/74fb68167525/nihms-2031133-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/dc11fd9bc90a/nihms-2031133-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/626b97a36ba2/nihms-2031133-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4387/11565437/abd5eed7d9ff/nihms-2031133-f0004.jpg

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本文引用的文献

1
The clock in growing hyphae and their synchronization in Neurospora crassa.在粗糙脉孢菌中生长的菌丝体及其同步时钟。
Commun Biol. 2024 Jun 18;7(1):735. doi: 10.1038/s42003-024-06429-6.
2
The EMBL-EBI Job Dispatcher sequence analysis tools framework in 2024.2024 年 EMBL-EBI 作业调度程序序列分析工具框架
Nucleic Acids Res. 2024 Jul 5;52(W1):W521-W525. doi: 10.1093/nar/gkae241.
3
The conserved domain database in 2023.2023 年的保守域数据库。
Nucleic Acids Res. 2023 Jan 6;51(D1):D384-D388. doi: 10.1093/nar/gkac1096.
4
Highly accurate protein structure prediction with AlphaFold.利用 AlphaFold 进行高精度蛋白质结构预测。
Nature. 2021 Aug;596(7873):583-589. doi: 10.1038/s41586-021-03819-2. Epub 2021 Jul 15.
5
PRD-2 directly regulates and counteracts nonsense-mediated decay in the Neurospora circadian clock.PRD-2 直接调控并拮抗 Neurospora 生物钟中的无意义介导的衰变。
Elife. 2020 Dec 9;9:e64007. doi: 10.7554/eLife.64007.
6
Spiral-based microfluidic device for long-term time course imaging of Neurospora crassa with single nucleus resolution.基于螺旋的微流控装置，用于对粗糙脉孢菌进行具有单核分辨率的长期时程成像。
Fungal Genet Biol. 2016 Sep;94:11-4. doi: 10.1016/j.fgb.2016.06.004. Epub 2016 Jun 21.
7
Circadian rhythms. Decoupling circadian clock protein turnover from circadian period determination.昼夜节律。使昼夜节律钟蛋白周转与昼夜节律周期测定脱钩。
Science. 2015 Jan 30;347(6221):1257277. doi: 10.1126/science.1257277.
8
Neurospora crassa: looking back and looking forward at a model microbe.粗糙脉孢菌：回顾与展望一种模式微生物
Am J Bot. 2014 Dec;101(12):2022-35. doi: 10.3732/ajb.1400377. Epub 2014 Nov 16.
9
Probing the growth dynamics of Neurospora crassa with microfluidic structures.用微流控结构探测粗糙脉孢菌的生长动态。
Fungal Biol. 2011 Jun;115(6):493-505. doi: 10.1016/j.funbio.2011.02.003. Epub 2011 Feb 24.
10
Visualization of F-actin localization and dynamics with live cell markers in Neurospora crassa.利用活细胞标记物在粗糙脉孢菌中可视化 F-肌动蛋白的定位和动态变化。
Fungal Genet Biol. 2010 Jul;47(7):573-86. doi: 10.1016/j.fgb.2010.03.004. Epub 2010 Mar 17.