Shija Vicent Michael, Zakaria Glory Emanuel, Amoah Kwaku, Yi Li, Huang Junwei, Masanja Fortunatus, Yong Zhong, Cai Jia
College of Fishery Guangdong Ocean University, Zhanjiang 524088, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China.
Aquac Nutr. 2024 Aug 12;2024:4253969. doi: 10.1155/2024/4253969. eCollection 2024.
In the present study, we investigated the effect of dietary supplementation with the probiotic AV5 (OR647358) on the growth, serum and mucus immune responses, metabolomics, and lipid metabolism of . Fishes (27.2 ± 1.7 g and 9.0 ± 1.2 cm) were fed three distinct meals: a commercial diet (control-GC) and two treatment diets supplemented with probiotics at 10 (G1) and 10 cfu/g (G2), respectively, for 30 days. In the G2 group, the final weight, specific growth rate, weight gain rate, survival rate, and feed conversion ratio of the fish were significantly improved ( < 0.05). Lysozyme, myeloperoxidase, and alkaline phosphatase activities in the mucus of fish were significantly higher ( < 0.05) in the G1 and G2 groups. The serum total protein, superoxide dismutase, glutathione peroxidase, reactive oxygen species, and reactive nitrogen species levels were noticeably higher ( < 0.05) in fish fed G1 and G2. In addition, in the G1 and G2 groups, higher levels of enzymes involved in lipid metabolism, such as pyruvate kinase, 2-hydroxyethyl-ThPP, and dihydrolipoamide dehydrogenase, were increased. Distal gastrointestinal metabolites, such as glycerophospholipids and histidine, were observed. These findings strongly indicate that incorporating AV5 at 10 cfu/g into commercial feeds positively influences fish growth, immunity, and lipid metabolism.
在本研究中，我们调查了日粮添加益生菌AV5（OR647358）对鱼类生长、血清和黏液免疫反应、代谢组学以及脂质代谢的影响。将体重为（27.2±1.7克）、体长为（9.0±1.2厘米）的鱼分为三组，分别投喂三种不同的饲料：一种商业饲料（对照-GC）和两种添加益生菌的饲料，添加量分别为10⁶（G1）和10⁷cfu/g（G2），持续投喂30天。在G2组中，鱼的终末体重、特定生长率、增重率、存活率和饲料转化率均显著提高（P<0.05）。G1组和G2组鱼黏液中的溶菌酶、髓过氧化物酶和碱性磷酸酶活性显著更高（P<0.05）。投喂G1和G2饲料的鱼血清总蛋白、超氧化物歧化酶、谷胱甘肽过氧化物酶、活性氧和活性氮水平明显更高（P<0.05）。此外，在G1和G2组中，参与脂质代谢的酶，如丙酮酸激酶、2-羟乙基硫胺焦磷酸和二氢硫辛酰胺脱氢酶的水平升高。还观察到远端胃肠道代谢产物，如甘油磷脂和组氨酸。这些发现有力地表明，在商业饲料中添加10⁷cfu/g的AV5对鱼类生长、免疫力和脂质代谢有积极影响。
