Xiao Liuyue, Hou Jiaojiao, Chen Ping, Tan Xiao, Qin Xiangxiang, Nie Jing, Zhu Hua, Zhong Shian
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
GuangXi University of Chinese Medicine, Nanning 530200, China.
Int J Biol Macromol. 2024 Dec;283(Pt 2):137382. doi: 10.1016/j.ijbiomac.2024.137382. Epub 2024 Nov 16.
A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 10 Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1] → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.
采用微波辅助法从广东石豆兰中提取多糖，并通过正交试验确定最佳提取条件。纯化后的多糖组分BKP-1在红外光谱上具有明显的多糖特征结构，主要由木糖和葡萄糖醛酸以0.568:0.432的比例组成。其分子量分布（多分散指数为0.58）均匀，为1.92×10 Da。BKP-1的主要结构骨架为→4)-β-D-木糖基-(1→[4)-β-D-木糖基-(1]→4)-α-D-葡萄糖醛酸基-(1→[4)-α-D-葡萄糖醛酸基-(1]→。BKP-1能够清除一定量的自由基，其中对羟基自由基的清除能力最佳，清除率为61.46%。此外，BKP-1在浓度为125μg/mL时能显著调节促炎和抗炎细胞因子的分泌。值得注意的是，BKP-1发挥抑制作用的机制与调节NF-κB信号通路有关。
