用于生物发光 RNA 追踪的模块化平台。
A modular platform for bioluminescent RNA tracking.
机构信息
Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA.
出版信息
Nat Commun. 2024 Nov 18;15(1):9992. doi: 10.1038/s41467-024-54263-5.
A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale.
要全面了解 RNA 生物学，就需要能够在体内追踪转录本的方法。常见的策略依赖于荧光探针，但由于需要激发光和组织自发荧光，这些探针在灵敏度、动态范围和检测深度方面都受到限制。为了克服这些挑战，我们报告了一种用于 RNA 连续成像的生物发光平台。这些 RNA 标签经过设计，在转录时会招募发光的荧光素酶片段（称为 RNA 灯笼）。在细胞和活体动物中，都观察到 RNA 靶标产生稳健的光子。重要的是，与需要多个重复的荧光平台相比，仅需一个标签拷贝即可进行灵敏检测，这形成了鲜明的对比。总的来说，这项工作为从微观到宏观尺度可视化 RNA 动态提供了一个基础平台。
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