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用于改善[具体生物]中第二代燃料乙醇生产的工程化转录调控网络

Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in .

作者信息

Sun Dongming, Wu Longhao, Lu Xiaocong, Li Chenhao, Xu Lili, Li Hongxing, He Deyun, Yu Aiqun, Yu Tao, Zhao Jianzhi, Tang Hongting, Bao Xiaoming

机构信息

Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, 3501 Daxue Road, Jinan, 250353, China.

Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes for Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

出版信息

Synth Syst Biotechnol. 2024 Oct 28;10(1):207-217. doi: 10.1016/j.synbio.2024.10.006. eCollection 2025.

DOI:10.1016/j.synbio.2024.10.006
PMID:39558946
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11570414/
Abstract

Presently, demonstrates proficient co-fermentation of glucose and xylose, marking a significant advancement in second-generation fuel ethanol production. However, the presence of high concentrations of inhibitors in industrial lignocellulose hydrolysates and post-glucose effect caused by glucose consumption hinders severely impedes yeast robustness and xylose utilization for ethanol fermentation. Even worse, the antagonism between xylose utilization ability and strain robustness was observed, which proposes a difficult challenge in the production of second-generation fuel ethanol by . This review introduces the effect of engineering transcriptional regulatory networks on enhancing xylose utilization, improving strain robustness, alleviating antagonism between xylose utilization and strain robustness, and reducing post-glucose effect. Additionally, we provide an outlook on the developmental trends in this field, offering insights into future directions for increasing the production of second-generation fuel ethanol in .

摘要

目前，已证明其能高效共发酵葡萄糖和木糖，这标志着第二代燃料乙醇生产取得了重大进展。然而，工业木质纤维素水解产物中高浓度抑制剂的存在以及葡萄糖消耗引起的葡萄糖后效应严重阻碍了酵母的稳健性和木糖用于乙醇发酵的利用率。更糟糕的是，观察到木糖利用能力与菌株稳健性之间的拮抗作用，这给通过……生产第二代燃料乙醇带来了艰巨挑战。本综述介绍了工程转录调控网络在提高木糖利用率、改善菌株稳健性、缓解木糖利用与菌株稳健性之间的拮抗作用以及降低葡萄糖后效应方面的作用。此外，我们对该领域的发展趋势进行了展望，为提高……中第二代燃料乙醇产量的未来方向提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a14/11570414/daed68647f6f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a14/11570414/0c453a6d2543/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a14/11570414/daed68647f6f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a14/11570414/0c453a6d2543/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a14/11570414/daed68647f6f/gr2.jpg

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Microorganisms. 2024 Jul 25;12(8):1526. doi: 10.3390/microorganisms12081526.
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Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.氧合作用影响嗜孢酵母属和休哈塔假丝酵母属酵母中的木糖发酵和基因表达。
Biotechnol Biofuels Bioprod. 2024 Feb 7;17(1):20. doi: 10.1186/s13068-024-02467-8.
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PKA-Msn2/4-Shy1 cascade controls inhibition of proline utilization under wine fermentation models.
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PKA-Msn2/4-Shy1 级联反应控制葡萄酒发酵模型中脯氨酸利用的抑制。
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Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate.从木低聚糖和醋酸盐到第二代乙醇生产的酿酒酵母的代谢工程。
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