Viswanathan Anitha Kuttae, Krishnan Rajkumar
Department of Prosthodontics, SRM Institute of Science and Technology, SRM dental college, Ramapuram campus, Chennai, TamilNadu, India, 600089.
Department of Oral Pathology, SRM Institute of Science and Technology, SRM dental college, Ramapuram campus, Chennai, TamilNadu, India, 600089.
J Oral Biol Craniofac Res. 2024 Nov-Dec;14(6):815-824. doi: 10.1016/j.jobcr.2024.10.014. Epub 2024 Nov 4.
The objective of the study was to determine the antimicrobial efficacy of Lavandula angustifolia (True Lavender extract) towards Candida albicans, Streptococcus mutans, Staphylococcus aureus pathogens. Varying proportions of the extract was incorporated into polymethyl methacrylate (PMMA) denture base resin and the anti-adherent effect was investigated.
An in-vitro study was performed after pure extract was obtained from Lavandula angustifolia (LA) flowers using a solvent based hot extraction process. Chromatographic analysis and computational molecular docking were done to analyze its phytoconstituents with potential target-ligand bond. Tests of antimicrobial susceptibility, minimum inhibitory concentration, minimum bactericidal and fungicidal dosages and in-vitro cytotoxicity were performed. Different proportions of LA extract (0, .5, 1.0, 1.5, and 2.0 %) were added to PMMA resin to assess anti-adherence property. Obtained data were statistically analyzed with One-way ANOVA followed by Tukey post-hoc tests.
The reports revealed significant antimicrobial susceptibility against the test pathogens compared to control drugs (P > 0.05). A minimum concentration of .02 mg of lavender extract inhibited microbial growth with low cytotoxicity (P < 0.05). The highest anti-adherent activity was observed in the .5 % LA-incorporated PMMA resin group (P = 0.0001).
Incorporating lavender extract into denture base resin demonstrated promising antimicrobial properties. This investigation encourages further research to understand its effects on mechanical and physical properties of reinforced dental resins.
本研究的目的是确定狭叶薰衣草（纯薰衣草提取物）对白色念珠菌、变形链球菌、金黄色葡萄球菌病原体的抗菌效果。将不同比例的提取物加入聚甲基丙烯酸甲酯（PMMA）义齿基托树脂中，并研究其抗粘附作用。
采用溶剂热萃取法从狭叶薰衣草（LA）花中获得纯提取物后进行体外研究。进行色谱分析和计算分子对接，以分析其具有潜在靶标-配体键的植物成分。进行了抗菌药敏试验、最低抑菌浓度、最低杀菌和杀真菌剂量以及体外细胞毒性试验。将不同比例的LA提取物（0%、0.5%、1.0%、1.5%和2.0%）添加到PMMA树脂中，以评估抗粘附性能。获得的数据采用单因素方差分析，然后进行Tukey事后检验进行统计学分析。
报告显示，与对照药物相比，对测试病原体具有显著的抗菌药敏性（P>0.05）。最低浓度为0.02mg的薰衣草提取物可抑制微生物生长，且细胞毒性较低（P<0.05）。在添加0.5%LA的PMMA树脂组中观察到最高的抗粘附活性（P=0.0001）。
将薰衣草提取物加入义齿基托树脂中显示出有前景的抗菌性能。本研究鼓励进一步研究，以了解其对增强牙科树脂机械和物理性能的影响。
