Rezghiyan Ayyub, Esmaeili Hassan, Farzaneh Mohsen, Rezadoost Hassan
Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, 1983969411, Tehran, Iran.
Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, 1983969411, Tehran, Iran.
Plant Physiol Biochem. 2024 Dec;217:109298. doi: 10.1016/j.plaphy.2024.109298. Epub 2024 Nov 15.
Different practical approaches have been employed to attenuate the destructive impacts of water deficit stress on plants, such as utilization of humic acid, salicylic acid, algae extract, mulching, and microorganisms, as well as silicon application. Nanosilicon significantly moderates the ruinous effects of abiotic and biotic stress in plants through some physiological processes. In this study, the interaction effect of drought stress and nanosilicon on phytochemical and physiological characteristics of hemp (Cannabis sativa L.) was investigated, wherein the four-week-old seedlings were subjected to irrigation treatments at four levels, including 100% (control), 80% (mild stress), 60% (moderate stress), and 40% (severe stress) of field capacity and nanosilicon at three concentrations (0, 0.5, and 1.5 mM) was foliar applied every 10 days in a factorial completely randomized design experiment with three replications for 30 days. Phytochemical and physiological analyses such as photosynthetic pigments, total phenolic and flavonoid content, and antioxidant enzyme activities were conducted. The results indicated that the highest content of Cannabidiol and Tetrahydrocannabinol was achieved using 1.5 mM (1.89%) and 0.5 mM (0.63%) nanosilicon treatments, respectively, under moderate stress. The plants subjected to severe drought stress without nanosilicon application displayed the lowest values of chlorophyll a (0.50 mg/g FW) and b (0.20 mg/g FW). The use of nanosilicon excited the activation of antioxidant enzymes, wherein the plants treated with nanosilicon and drought stress exhibited significantly higher SOD, POD, and APX activities compared to the control. Under all drought stress levels, foliar application of nanosilicon at the highest concentration decreased proline content. The results proposed that the application of 1.5 mM nanosilicon, as a more efficient concentration, improved drought tolerance in hemp plants.
人们采用了不同的实用方法来减轻水分亏缺胁迫对植物的破坏性影响，例如使用腐殖酸、水杨酸、藻类提取物、覆盖物和微生物，以及施用硅。纳米硅通过一些生理过程显著减轻了非生物和生物胁迫对植物的有害影响。在本研究中，研究了干旱胁迫和纳米硅对大麻（Cannabis sativa L.）植物化学和生理特性的交互作用，其中对四周龄的幼苗进行了四个水平的灌溉处理，包括田间持水量的100%（对照）、80%（轻度胁迫）、60%（中度胁迫）和40%（重度胁迫），并以完全随机区组设计实验，每10天叶面喷施三种浓度（0、0.5和1.5 mM）的纳米硅，重复三次，持续30天。进行了光合色素、总酚和黄酮含量以及抗氧化酶活性等植物化学和生理分析。结果表明，在中度胁迫下，分别使用1.5 mM（1.89%）和0.5 mM（0.63%）纳米硅处理时，大麻二酚和四氢大麻酚的含量最高。未施用纳米硅的重度干旱胁迫植物叶绿素a（0.50 mg/g鲜重）和叶绿素b（0.20 mg/g鲜重）的值最低。纳米硅的使用激发了抗氧化酶的活性，与对照相比，纳米硅处理和干旱胁迫处理的植物超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶活性显著更高。在所有干旱胁迫水平下，叶面喷施最高浓度的纳米硅可降低脯氨酸含量。结果表明，施用1.5 mM纳米硅作为更有效的浓度可提高大麻植物的耐旱性。
