Rezghiyan Ayyub, Esmaeili Hassan, Farzaneh Mohsen
Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, 1983969411, Iran.
Sci Rep. 2025 Jan 27;15(1):3400. doi: 10.1038/s41598-025-87611-6.
Various practical strategies have been employed to mitigate the detrimental effects of water deficit stress on plants such as application of nano-stimulants. Nanosilicon plays a crucial role in alleviating the deleterious impacts of both abiotic and biotic stresses in plants by modulating various phyto-morphological and physiological processes. This study aimed to examine the combined effects of drought stress and nanosilicon application on the morphological traits and essential oil content and compositions of hemp (Cannabis sativa L.), in which four-week-old seedlings were subjected to irrigation treatments at four levels, including 100% (control), 80% (mild stress), 60% (moderate stress) and 40% (severe stress) field capacity and nanosilicon at three concentrations (0, 0.5 and 1.5 mM) in a completely randomized factorial design experiment with three replications for 40 days. The results showed that the maximum plant height (109.07 cm), number of nodes (33.3), and number of flowering branches (29.4) were recorded under the treatment of 1.5 mM nanosilicon and 100% FC. The lowest fresh and dry weights of aerial parts were associated to the severe drought stress (40% FC) without nanosilicon application. The mild water stress (80% FC) combined with foliar application of 1.5 mM nanosilicon led to highest EO content (0.17%) compared with the other treatments. However, the highest content of cannabidiol in the essential oil was achieved in the severe water stress (40% FC) and treatment of 0.5 mM nanosilicon. The results showed that the application of nanosilicon improved the morphological characteristics and also changed the content and compositions of the hemp plants under drought stress conditions.
人们已经采用了各种实际策略来减轻水分亏缺胁迫对植物的不利影响，比如应用纳米刺激剂。纳米硅通过调节植物的各种形态和生理过程，在减轻非生物和生物胁迫对植物的有害影响方面发挥着关键作用。本研究旨在考察干旱胁迫和纳米硅施用对大麻（Cannabis sativa L.）形态特征、精油含量及成分的综合影响，在完全随机析因设计试验中，对四周龄的幼苗进行四种水平的灌溉处理，包括100%（对照）、80%（轻度胁迫）、60%（中度胁迫）和40%（重度胁迫）的田间持水量，并设置三种浓度（0、0.5和1.5 mM）的纳米硅，重复三次，处理40天。结果表明，在1.5 mM纳米硅和100%田间持水量的处理下，植株高度最高（109.07 cm）、节数（33.3）和开花枝数（29.4）最多。地上部分的最低鲜重和干重与不施用纳米硅的重度干旱胁迫（40%田间持水量）有关。与其他处理相比，轻度水分胁迫（80%田间持水量）结合叶面喷施1.5 mM纳米硅导致精油含量最高（0.17%）。然而，在重度水分胁迫（40%田间持水量）和0.5 mM纳米硅处理下，精油中大麻二酚的含量最高。结果表明，纳米硅的施用改善了干旱胁迫条件下大麻植株的形态特征，也改变了其精油的含量和成分。
