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CYP4BN4v7 调控豆象种群密度依赖性卵母细胞成熟率。

CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles.

机构信息

School of Life Sciences, Guizhou Normal University, Gui'an, Guizhou, China.

Qiannan Normal University for Nationalities, Duyun, Guizhou, China.

出版信息

Sci Rep. 2024 Nov 19;14(1):28574. doi: 10.1038/s41598-024-79866-2.

DOI:10.1038/s41598-024-79866-2
PMID:39562601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11576951/
Abstract

The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L). A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes. Gene duplication might occur in CYP9Z4-like, CYP345A1-like, CYP345A2-like, CYP349A1-like, CYP349A2-like, and CYP4BN4-like. Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H. Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP. CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen. Its mRNA levels in the head, thorax, and abdomen were greater in H than in L. After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased. Moreover, the expression level of insulin-like peptide 1 (ILP1), which plays a vital role in regulating terminal oocyte development, decreased in the head. In conclusion, CYP4BN4v7-like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1.

摘要

豆象（Callosobruchus maculatus）明显表现出种群密度依赖的多态性。细胞色素 P450（CYP）参与昆虫许多生理活动。然而，CYP 在种群密度依赖的多态性中的作用尚不清楚。高密度个体（H）的终末卵母细胞成熟率快于低密度个体（L）。从转录组和基因组数据中鉴定出 56 个 CYP 样基因，包括 7 个族 2 CYP 样基因、7 个线粒体 CYP 样基因、19 个族 3 CYP 样基因和 23 个族 4 CYP 样基因。CYP9Z4 样、CYP345A1 样、CYP345A2 样、CYP349A1 样、CYP349A2 样和 CYP4BN4 样可能发生基因复制。H 中分别上调和下调了 13 个和 2 个 CYP 样基因。在这些 CYP 样基因中，CYP4BN4v7 样是最丰富的 CYP。CYP4BN4v7 样在头部的表达高于胸部和腹部。其头部、胸部和腹部的 mRNA 水平在 H 中大于 L。RNA 干扰降低其 mRNA 水平后，终末卵母细胞成熟率降低。此外，在头部，对调节终末卵母细胞发育至关重要的胰岛素样肽 1（ILP1）的表达水平降低。总之，CYP4BN4v7 样通过调节 ILP1 的表达来调节种群密度依赖的终末卵母细胞成熟率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/5c6bb7b77745/41598_2024_79866_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/53e9848d2f6b/41598_2024_79866_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/740583e1a6c5/41598_2024_79866_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/21252bd43dc6/41598_2024_79866_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/7e6480bb0128/41598_2024_79866_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/5c6bb7b77745/41598_2024_79866_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/53e9848d2f6b/41598_2024_79866_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/740583e1a6c5/41598_2024_79866_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/21252bd43dc6/41598_2024_79866_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/7e6480bb0128/41598_2024_79866_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/481c/11576951/5c6bb7b77745/41598_2024_79866_Fig5_HTML.jpg

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