Centre de Recherche des Cordeliers, INSERM, Université Paris Cité, Sorbonne Université, Physiopathology of ocular diseases: Therapeutic innovations, Paris, France.
Ophthalmopole Cochin University Hospital, Assistance Publique-Hôpitaux de Paris, France.
Mol Vis. 2024 Mar 30;30:211-218. eCollection 2024.
To investigate systemic and ocular toll-like receptor (TLR)-4 expression and its association with oxidative stress markers in ocular rosacea (OR).
This prospective study included 40 patients with rosacea with ocular involvement and 20 healthy volunteers. Tear break-up time (TBUT), Schirmer test, meibomoscore, and ocular surface disease index (OSDI) scores were estimated for all participants. TLR-4 expression in conjunctival epithelium and peripheral blood mononuclear cells was quantified using real-time polymerase chain reaction (RT-PCR). In the tears and serum samples of all participants, antioxidant status (TAS), total oxidant status (TOS), and arylesterase (ARE) activation levels were measured using a fully automated spectrophotometric method, and the oxidative stress index (OSI) was calculated.
TLR-4 expression levels and oxidative stress status (TOS and OSI values) were significantly higher ( < 0.01), and antioxidant status (TAS and ARE values) were significantly lower ( < 0.01) in both ocular and blood samples of patients with OR compared with those in controls. A significant positive correlation was found between the ocular and blood values in all parameters ( < 0.05). According to the clinical associations of these results, we found negative correlations between TLR-4, OSI, and TBUT and between TLR-4 and Schirmer, whereas a positive correlation was observed between TLR-4, OSI, and meiboscore and between TLR-4, OSI, and OSDI ( < 0.05). No correlation was found between the OSI and Schirmer results ( = 0.92).
TLR-4 and oxidative stress both play important roles in OR pathophysiology and are closely related to clinical findings.
研究眼型酒渣鼻（OR）中系统性和眼内 toll 样受体（TLR）-4 的表达及其与氧化应激标志物的关系。
本前瞻性研究纳入了 40 例伴有眼受累的酒渣鼻患者和 20 名健康志愿者。对所有参与者进行泪膜破裂时间（TBUT）、Schirmer 试验、睑板腺评分和眼表疾病指数（OSDI）评分。使用实时聚合酶链反应（RT-PCR）定量评估结膜上皮和外周血单个核细胞中 TLR-4 的表达。在所有参与者的泪液和血清样本中，使用全自动分光光度法测量抗氧化状态（TAS）、总氧化状态（TOS）和芳酯酶（ARE）激活水平，并计算氧化应激指数（OSI）。
与对照组相比，OR 患者的眼内和血液样本中 TLR-4 表达水平和氧化应激状态（TOS 和 OSI 值）显著升高（<0.01），而抗氧化状态（TAS 和 ARE 值）显著降低（<0.01）。所有参数的眼内和血液值之间均存在显著正相关（<0.05）。根据这些结果的临床相关性，我们发现 TLR-4、OSI 和 TBUT 之间以及 TLR-4 和 Schirmer 之间呈负相关，而 TLR-4、OSI 和睑板腺评分之间以及 TLR-4、OSI 和 OSDI 之间呈正相关（<0.05）。OSI 和 Schirmer 结果之间无相关性（=0.92）。
TLR-4 和氧化应激在 OR 发病机制中均发挥重要作用，且与临床发现密切相关。
