Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China.
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Carbohydr Polym. 2025 Jan 15;348(Pt B):122900. doi: 10.1016/j.carbpol.2024.122900. Epub 2024 Oct 21.
Diabetic foot ulcers (DFUs) as a nonhealing wound remain a clinical challenge, and the development of pro-healing and cost-effective drugs is in urgent need. Herein, we reported a novel galactosylated glycosaminoglycan (GAG) from the snail Helix lucorum, as an effective pro-healing compound. The snail GAG is composed of a heparan sulfate-like main chain and galactose side chains at C-3 of GlcNAc residue. Its main chain has a repeating disaccharide structure of → 4)-α-D-GlcNAc-(1 → 4)-α-L-IdoA(1 →. This is the first example of glycosaminoglycan with galactose branches from mollusks. Pharmacological experiments showed that the H. lucorum GAG significantly promoted skin wound healing in both healthy and diabetic mice by accelerating granulation tissue regeneration, angiogenesis, and collagen deposition. The distinctive galactosylated substitution may play an important role on its pro-healing activity. Our discovery enriches the diversity of non-anticoagulant heparan sulfate-like glycosaminoglycans, and provides a potential candidate of pro-healing drug for treating diabetic wound.
糖尿病足溃疡（DFUs）作为一种难以愈合的伤口仍然是临床面临的挑战，因此迫切需要开发具有促愈作用且经济有效的药物。在此，我们报道了一种来自蜗牛赫蕉（Helix lucorum）的新型半乳糖化糖胺聚糖（GAG），它是一种有效的促愈化合物。该蜗牛 GAG 由肝素硫酸盐样主链和 GlcNAc 残基 C-3 上的半乳糖侧链组成。其主链具有重复的二糖结构→4）-α-D-GlcNAc-（1→4）-α-L-IdoA（1→。这是首例来自软体动物的具有半乳糖支链的糖胺聚糖。药理学实验表明，赫蕉 GAG 通过加速肉芽组织再生、血管生成和胶原沉积，显著促进了健康和糖尿病小鼠的皮肤伤口愈合。独特的半乳糖化取代可能对其促愈活性起着重要作用。我们的发现丰富了非抗凝肝素硫酸盐样糖胺聚糖的多样性，并为治疗糖尿病伤口提供了一种有潜力的促愈药物候选物。
