Section for Sports Traumatology, Department of Orthopedic Surgery, Copenhagen University Hospital Bispebjerg, Hospital Bispebjerg, 2400, Copenhagen NV, Denmark.
Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
Cell Tissue Bank. 2024 Nov 23;26(1):1. doi: 10.1007/s10561-024-10151-2.
It is common practice that allograft tissues for knee multiligament reconstruction, meniscus transplantation, cartilage replacement and other advanced procedures are made available through commercial banks. The aim was to present the 10-year experience with a local, non-commercial tissue bank, established in 2014. The allograft bank was connected to an existing organ donor program. Following organ procurement, the musculoskeletal tissue is removed and stored immediately, fresh frozen to - 80 degrees Celsius (except hyaline cartilage, which is stored at 5 degrees Celsius). The donor is tested for contagious disease and the grafts for bacteria. When all results are negative, the grafts are released. When thawed before use a swap is cultured. Consecutive, prospectively collected data were analyzed. There were 31 donations, resulting in 1160 grafts. Only 40 grafts (3.4%) had a positive bacteria culture and were discarded. 552 recipients have been treated by use of these allografts. All grafts had negative bacterial cultures in swaps obtained before thawing, and there were no recorded transplantation related complications. The expenses for local grafts were 10-15% of the costs for grafts obtained from foreign banks. Through the local donation program, it was possible to establish a tissue bank with controlled quality grafts at minimal costs, minimizing the need for transportation of frozen grafts retrieved in other countries. Centers for specialized orthopedic surgery using allograft tissue can be self-providing and reduce costs by retrieving and handling allograft tissues locally.
异体组织库常用于膝关节多韧带重建、半月板移植、软骨置换和其他高级手术，这些组织通常由商业银行提供。本研究旨在介绍 2014 年建立的、非商业化的本地组织库的 10 年经验。该组织库与现有的器官捐献项目相连接。在获取器官后，立即将其肌肉骨骼组织取出并冷藏保存，新鲜冷冻至-80°C（透明软骨除外，其在 5°C 下储存）。对捐献者进行传染病检测，对移植物进行细菌检测。所有结果均为阴性时，移植物将被放行。在使用前解冻时，会进行一次交换培养。分析了连续的、前瞻性收集的数据。共有 31 次捐赠，产生了 1160 个移植物。只有 40 个移植物（3.4%）的细菌培养呈阳性，被丢弃。552 名患者使用了这些同种异体移植物进行治疗。在解冻前获得的交换物中，所有移植物的细菌培养均呈阴性，且没有记录到与移植相关的并发症。本地移植物的费用为从外国银行获得的移植物费用的 10-15%。通过本地捐赠计划，我们以最小的成本建立了一个组织库，该组织库的移植物质量得到了控制，最大限度地减少了从其他国家运输冷冻移植物的需求。使用同种异体组织的专业骨科中心可以自行检索和处理本地的同种异体组织，从而降低成本。
