College of Chemistry and Material, Jiangxi Normal University, 99 Ziyang Road, Nanchang, 330022, PR China.
Guangzhou Customs District Technology Center, No.66 Huacheng Avenue, Zhujiang New City, Guangzhou, 510623, PR China.
Mikrochim Acta. 2024 Nov 23;191(12):757. doi: 10.1007/s00604-024-06827-x.
To improve the fluorescence properties of covalent organic framework (COF) materials, core-shell SiO@COF composite was prepared, in which SiO was used as inner substrate, and BMTH (2,5-bis (2-methoxyethoxy) p-phenylhydrazide) and HB (2-hydroxy-1,3,5-phenyltrialdehyde) acted as precursors to construct COF layer on the surface of SiO. Compared with COF, SiO@COF composite showed better dispersion property and improved fluorescence performance under the same experimental conditions. Using SiO@COF as a fluorescence probe, the fluorescence intensity was gradually decreased with the addition of Cu, while it was restored by introducing Cys or Hcy, realizing "ON-OFF-ON" fluorescence sensing detection. Other amino acids with five times the concentration of Cys showed little effect on the determination of Cys or Hcy. The SiO@COF-Cu probe exhibited ultrahigh sensitivity and good selectivity for the detection of Cys and Hcy, with a detection range of 0.23 to 250.0 µM for Cys and 0.31 to 170.0 µM for Hcy. The detection limits (LOD = 3 σ/s) were 75 and 104 nM for Cys and Hcy, respectively. The system also showed a good recovery and low relative standard deviations in actual sample tests for Cys or Hcy, demonstrating its potential for practical applications.
为了改善共价有机骨架（COF）材料的荧光性能，制备了核壳结构的 SiO@COF 复合材料，其中 SiO 作为内芯，BMTH（2,5-双（2-甲氧基乙氧基）对苯二酰肼）和 HB（2-羟基-1,3,5-均苯三甲醛）作为前体，在 SiO 表面构建 COF 层。与 COF 相比，SiO@COF 复合材料在相同实验条件下具有更好的分散性能和改善的荧光性能。将 SiO@COF 用作荧光探针，随着 Cu 的加入，荧光强度逐渐降低，而通过引入 Cys 或 Hcy 可将其恢复，实现了“开-关-开”荧光传感检测。浓度是 Cys 五倍的其他五种氨基酸对 Cys 或 Hcy 的测定几乎没有影响。SiO@COF-Cu 探针对 Cys 和 Hcy 的检测具有超高的灵敏度和良好的选择性，Cys 的检测范围为 0.23 至 250.0 μM，Hcy 的检测范围为 0.31 至 170.0 μM。Cys 和 Hcy 的检测限（LOD=3 σ/s）分别为 75 和 104 nM。该系统在实际样品中测试 Cys 或 Hcy 时也表现出良好的回收率和低相对标准偏差，表明其具有实际应用的潜力。
