Clinical Laboratory, Henan Provincial People's Hospital, Fuwai Central China Cardiovascular Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan 451464, China.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
Anal Chim Acta. 2024 Dec 15;1332:343359. doi: 10.1016/j.aca.2024.343359. Epub 2024 Oct 23.
In this work, a self-powered microsensor platform based on enzyme biofuel cells (EBFCs) was developed for intelligent monitoring of disease markers miRNA-451. The cascade catalysis system constructed by using the strategy of enzyme-like ZIF-8 nanocapsule incorporation with biological enzymes, which could simultaneously take into account the specificity of biological enzymes and the high activity of nano-enzymes, significantly promoted the electron transfer between glucose and the bio-anode surface, and improved the sensitivity and stability of the sensing system. Meanwhile, the target-triggered hybridization chain reaction (HCR) amplification strategy to achieve exponential signal amplification based on accurate recognition, and jointly improve the detection sensitivity. As expected, the micro-sensor platform has a wide linear range of 0.5-1.0 fmol/L with a low limit of detection (LOD) of 0.13 fmol/L (S/N = 3) and exhibits excellent selectivity, reproducibility and stability in interference assays under optimal detection conditions. The designed self-powered system is simple to construct, easy to transport and the data transmission mode is intelligent and controllable, which is expected to be used in basic biochemical research, clinical diagnosis and environmental monitoring.
在这项工作中，开发了基于酶生物燃料电池 (EBFC) 的自供电微传感器平台，用于智能监测疾病标志物 miRNA-451。通过使用酶样 ZIF-8 纳米胶囊与生物酶结合的策略构建级联催化体系，同时考虑到生物酶的特异性和纳米酶的高活性，显著促进了葡萄糖与生物阳极表面之间的电子转移，提高了传感系统的灵敏度和稳定性。同时，基于准确识别的目标触发杂交链式反应 (HCR) 扩增策略实现了指数信号放大，共同提高了检测灵敏度。正如预期的那样，微传感器平台具有较宽的线性范围为 0.5-1.0 fmol/L，检测限 (LOD) 低至 0.13 fmol/L（S/N = 3），在最佳检测条件下的干扰测定中表现出优异的选择性、重现性和稳定性。设计的自供电系统结构简单，易于运输，数据传输方式智能可控，有望用于基础生化研究、临床诊断和环境监测。
