College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, Hunan, China.
Anal Chem. 2024 Oct 1;96(39):15780-15788. doi: 10.1021/acs.analchem.4c03854. Epub 2024 Sep 20.
A smartphone-mediated self-powered biosensor is fabricated for miRNA-141 detection based on the CRISPR/Cas12a cross-cutting technique and a highly efficient nanozyme. As a novel nanozyme and a signal-amplified coreaction accelerator, the AuPtPd@GDY nanozyme exhibits an excellent ability to catalyze cascade color reactions and high conductivity to enhance the electrochemical signal for miRNA-141 assays. After CRISPR/Cas12a cross-cutting of S2-glucose oxidase (S2-GOD), the electrochemical signal is weakened, and miRNA-141 is detected by monitoring the decrease in the signal. On the other hand, a cascade reaction among glucose, HO, and TMB is catalyzed by GOD and AuPtPd@GDY, respectively, resulting in a color change of the solution, which senses miRNA-141. The self-powered biosensor enables value-assisted and visual detection of miRNA-141 with limits of detection of 3.1 and 15 aM, respectively. Based on the dual-modal self-powered sensing system, a smartphone-mediated "all-in-one" biosensing chip is designed to achieve the real-time and intelligent monitoring of miRNA-141. This work provides a new approach to design multifunctional biosensors to realize the visualization and portable detection of tumor biomarkers.
一种基于 CRISPR/Cas12a 切割技术和高效纳米酶的智能手机介导自供电生物传感器被用于 miRNA-141 的检测。作为一种新型纳米酶和信号放大核心反应加速剂，AuPtPd@GDY 纳米酶表现出优异的催化级联显色反应的能力和高导电性，以增强用于 miRNA-141 分析的电化学信号。在 S2-葡萄糖氧化酶（S2-GOD）的 CRISPR/Cas12a 切割之后，电化学信号减弱，通过监测信号的减少来检测 miRNA-141。另一方面，葡萄糖、HO 和 TMB 之间的级联反应分别由 GOD 和 AuPtPd@GDY 催化，导致溶液颜色发生变化，从而感应 miRNA-141。自供电生物传感器能够实现 miRNA-141 的辅助值和可视化检测，检测限分别为 3.1 和 15 aM。基于双模态自供电传感系统，设计了一种智能手机介导的“一体化”生物传感芯片，实现了 miRNA-141 的实时和智能监测。这项工作为设计多功能生物传感器以实现肿瘤生物标志物的可视化和便携式检测提供了一种新方法。
