Bybordi Sara, Safa Pouya Baradaran, Eslami Sahand, Alipourpanahi Sara, Irani Mohammad
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Neuroscience, Faculty of Health Sciences, Yeditepe University, Istanbul, Turkey.
Int J Biol Macromol. 2024 Dec;283(Pt 4):137976. doi: 10.1016/j.ijbiomac.2024.137976. Epub 2024 Nov 23.
The use of biocompatible metal-organic frameworks (MOFs) and electrospun nanofibrous implants shows promise in preventing the recurrence of postsurgical glioblastoma. In this study, temozolomide (TMZ) and platinum‑gold nanorods (PtAu NRs) were encapsulated into the UiO-66-NH MOFs. These were then incorporated into the chitosan-grafted polycaprolactone (PCL) (core)/PCL (shell) nanofibers coated with PtAu NRs for extended release of TMZ during chemo-photothermal therapy against glioblastoma cells. The drug encapsulation efficiency, TMZ release, and in vitro cell viability were investigated for the MOFs, simple nanofibers, core-shell nanofibers, and MOFs-nanofibers. The extended release of TMZ occurred over 44 and 36 days from the core-shell nanofibers coated with PtAu NRs under NIR irradiation at pH values of 7.4 and 5, respectively. The maximum killing of U87 glioblastoma cells was 80.2 % using TMZ-Pt-Au-MOF-core-shell nanofibers coated with PtAu under NIR irradiation. The relative tumor size for the mice bearing glioblastoma and treated with pure core-shell nanofibers, TMZ-Pt-Au-MOF, and TMZ-Pt-Au-MOF-core-shell nanofibers coated with PtAu without NIR irradiation and with NIR irradiation was 4.12, 2.12, 1.65, 0.86, and 0.48, respectively, after 30 days. The synthesized MOF-core-shell nanofibers-Pt-Au NRs implantable device shows potential as a new approach for postsurgical glioblastoma treatment during chemo-photothermal therapy.
使用生物相容性金属有机框架（MOF）和电纺纳米纤维植入物在预防术后胶质母细胞瘤复发方面显示出前景。在本研究中，将替莫唑胺（TMZ）和铂 - 金纳米棒（PtAu NRs）封装到UiO - 66 - NH MOF中。然后将它们掺入接枝壳聚糖的聚己内酯（PCL）（核）/PCL（壳）纳米纤维中，该纳米纤维涂覆有PtAu NRs，以便在针对胶质母细胞瘤细胞的化学 - 光热疗法期间实现TMZ的缓释。研究了MOF、简单纳米纤维、核壳纳米纤维和MOF - 纳米纤维的药物包封效率、TMZ释放和体外细胞活力。在pH值为7.4和5的条件下，分别在近红外（NIR）照射下，涂覆有PtAu NRs的核壳纳米纤维在44天和36天内实现了TMZ的缓释。在NIR照射下，使用涂覆有PtAu的TMZ - Pt - Au - MOF - 核壳纳米纤维对U87胶质母细胞瘤细胞的最大杀伤率为80.2%。在30天后，对于患有胶质母细胞瘤并分别用纯核壳纳米纤维、TMZ - Pt - Au - MOF以及未进行NIR照射和进行NIR照射的涂覆有PtAu的TMZ - Pt - Au - MOF - 核壳纳米纤维治疗小鼠的相对肿瘤大小分别为4.12、2.12、1.65、0.86和0.48。合成的MOF - 核壳纳米纤维 - Pt - Au NRs可植入装置显示出作为化学 - 光热疗法期间术后胶质母细胞瘤治疗新方法的潜力。
